introduction: considering the role of hla matching in transplant outcome, the quality of hla-dr typing is clearly an important issue.in recent years serologic methods have been replaced with dna based typing methods.the main objective of this study was to compare hla-dr typing data obtained from existing serologic method with that obtained by the new pcr-ssp method. material and methods: fifty-...

BACKGROUND Polymerase chain reaction (PCR) has well established advantages over culture for diagnosis of herpes viruses, but its technical complexity has limited its widespread application. However, recent methodological advances have rendered PCR more applicable to routine practice. AIM To compare automated PCR with viral culture for diagnosis of genital herpes. METHODS We studied 236 pati...

The aim of the study was to detect HIV RNA in seropositive patients using RT-PCR method and thus, to establish PCR methodology in the routine laboratory works. The total of 33 examined persons were divided in two groups: 1) 13 persons seropositive for HIV; and 2) 20 healthy persons - randomly selected blood donors that made the case control group. The subjects age was between 25 and 52 years (a...

background infections caused by shigella are a major cause of diarrheal disease in the developing and developed countries. the present study was conducted to apply and evaluate arbitrarily primed pcr (ap-pcr) for investigation of genetic relatedness among the strains of shigella sonnei isolated from cases of acute diarrhea in tehran. patients and methods totally, 60 s. sonnei strains isolated f...

UNLABELLED We evaluate the elimination of the microscopic stage of conventional xenodiagnosis (XD) to optimize the parasitological diagnosis of Trypanosoma cruzi in chronic Chagas disease. To this purpose we applied under informed consent two XD cages to 150 Chilean chronic chagasic patients. The fecal samples (FS) of the triatomines at 30, 60 and 90 days post feeding were divided into two part...

The aim of this study was to validate the molecular genetic diagnosis of patients suspected of Fragile X Syndrome (FXS) in the Laboratory of Human Cytogenetics and Molecular Genetics (LaGene) of the Department of Health of the State of Goiás, using polymerase chain reaction (PCR). Thirty-five patients referred by public health doctors to LaGene, indicating clinical diagnosis of FXS, were select...

Abstract Surveillance for soil-transmitted helminths, strongyloidiasis, cryptosporidiosis, and giardiasis was conducted in Mississippi, USA. PCR performed on 224 fecal samples all helminths 370 only Necator americanus Strongyloides stercoralis identified 1 S. infection. Seroprevalences were 8.8% Toxocara, 27.4% Cryptosporidium, 5.7% Giardia, 0.2% parasites.