نتایج جستجو برای: pegfp

تعداد نتایج: 392  

Journal: :American journal of physiology. Renal physiology 2000
D H Sweet D S Miller J B Pritchard

The localization of organic cation transporter 2 (OCT2) within renal cells is the subject of considerable controversy, resulting in marked uncertainty as to its function. To resolve this issue, we made an OCT2/green fluorescent protein (GFP) fusion construct (rOCT2-GFP) and determined its localization within Xenopus laevis oocytes and renal cells using confocal microscopy. Oocytes expressing rO...

Journal: :iranian journal of basic medical sciences 0
raheleh halabian research center, iranian blood transfusion organization, tehran iran biotechnology department, tarbiyat modares university, tehran, iran mohamad hosein mohammadi research center, iranian blood transfusion organization, tehran iran mohammad salimi research center, iranian blood transfusion organization, tehran iran maryam amani research center, iranian blood transfusion organization, tehran iran amaneh mohammadi roushande department of anatomy, faculty of medicine, medical university of tabriz, tabriz, iran

objective(s) mesenchymal stem cells (mscs) are nonhematopoietic stromal cells that are capable of differentiating into and contribute to the regeneration of mesenchymal tissues. human mesenchymal stem cells (hmscs) are ideal targets in cell transplantation and tissue engineering. enhanced green fluorescent protein (egfp) has been an important reporter gene for gene therapy. the aim of this stud...

ژورنال: :پژوهش های آسیب شناسی زیستی 0
farahnaz motamedi sedeh ph.d. student, department of virology, faculty of medical science, tarbiat modares university, tehran, iran hooreih soleimanjahi associated professor, department of virology, faculty of medical science, tarbiat modares university, tehran, iran amirreza jalilian associated professor, department of nuclear medicine, nuclear agriculture department, nuclear science and technology research institute, tehran iran homayoon mahravani head of fmd department/ razi vaccine and serum research institute

هدف: هدف ساخت ناقل بیانی حاوی ژن vp1 ویروس تب برفکی سویه o جداسازی شده در ایران، بیان پروتئین و ارزیابی پاسخ‏های ایمنی در موش است. مواد و روش‏ها: سویه ویروس تب برفکی o از گوساله‏ای در شهر ری در سال 1386 جداسازی، سروتایپ و ژن vp1 این ویروس در ناقل ptz57r/t و ناقل بیانی pcdna3.1+ الحاق شد. برای بررسی بیان پروتئین، ژن vp1 بدون کدون خاتمه در ناقل pegfp-n1 بالادست ژن gfp کلون شد. ناقل‏های pegfp-n1-v...

Journal: :acta medica iranica 0
gholam reza mobini department of molecular medicine, school of advanced technologies in medicine, tehran university of medical sciences, tehran, iran. mohammad hossein ghahremani department of pharmacology and toxicology, school of pharmacy, tehran university of medical sciences, tehran, iran. saeid amanpour cancer models research center, cancer institute of iran, tehran university of medical sciences, tehran, iran. ahmad reza dehpour department of pharmacology, school of medicine, tehran university of medical sciences, tehran, iran. and experimental medicine research center, tehran university of medical sciences, tehran, iran. abolfazl akbari department of molecular medicine, school of advanced technologies in medicine, tehran university of medical sciences, tehran, iran. and colorectal research center, rasoul-e-akram hospital, iran university of medical sciences, tehran, iran. seyed mojtaba hoseiniharouni department of molecular medicine, school of advanced technologies in medicine, tehran university of medical sciences, tehran, iran.

a member of homeodomain protein namely tgif2lx has been implicated as a tumor suppressor gene in human malignancy as well as in spermatogenesis. however, to our knowledge, dynamic functional evidence of the tgif2lx has not yet been provided. the aim of the present study was to investigate the human tgif2lx target gene(s) using a cdna-aflp as a differential display method. a pegfp-tgif2lx constr...

پایان نامه :وزارت علوم، تحقیقات و فناوری - دانشگاه پیام نور - دانشگاه پیام نور استان البرز - دانشکده کشاورزی 1389

سل یکی از معضلات بهداشتی و پزشکی جامعه بشری است، که از سالیان دور تا کنون باعث خسارات جانی و مالی فراوانی برای نسل بشر شده است.و علی رغم تمام تلاشهای صورت گرفته برای مقابله با این بیماری هنوز ان را به عنوان یکی از مهمترین علل مرگ و میر در دنیا می دانند که سالیانه 2 میلیون نفر را در دنیا به کام مرگ می فرستد. یکی از مهمترین پروتئینهای ایمنی زا در مایکوباکتریوم توبرکلوزیسesat-6است،که یک پلی پپتید...

ژورنال: Vaccine Research 2017
Arashkia , A, Azadmanesh , K, Barzegar , H, Langroudi , L, Sharifi , H,

Introduction: Human papillomavirus (HPV) is the main causative agent of cervical cancer worldwide leading to a big health problem, especially in the developing countries. Among 14 common high-risk genotypes, HPV16 accounts for more than 50% of all cervical cancers. The current prophylactic vaccines against HPV infection are based on L1 protein. Due to some drawbacks in the current vaccines such...

Journal: :American journal of physiology. Renal physiology 2015
A Blumenthal J Giebel G Warsow L Li R Ummanni S Schordan E Schordan P Klemm N Gretz K Endlich N Endlich

Elevated glomerular pressure represents a high risk for the development of severe kidney diseases and causes an increase in mechanical load to podocytes. In this study, we investigated whether mechanical stress alters gene expression in cultured podocytes using gene arrays. We found that tetraspanin CD9 is significantly upregulated in cultured podocytes after mechanical stress. The differential...

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