Lipoprotein lipase is found in many species and Ussue types 11) and is the pivotal enzyme in controlling lipid flux between plasma triacylglycerol rich lipoprotein particles and the tissues. Because tissue LPL activities alter with the physiological state of an organism, it is of great commercial and economic importance, and an understanding of the way factors interact to regulate its expressio...

       Cholera toxin B subunit (CtxB) is a homopantameric, nontoxic subunit of cholera toxin that is responsible for its binding to the cell and has been known as a mucosal adjuvant for vaccines that could increase homoral and mocusal immunity response. In this work, the CtxB gene was fused to the StxB gene from Shigella dysenteriae type I a vaccine antigen candidate against t...

The insertion of multiple copies of DNA elements into plasmid vectors is frequently used to amplify the functions of the element. For example, tandem repeat elements can generate a hyperactive bait in yeast one-hybrid systems (3), and multiple copies of enhancer elements can greatly increase promoter strength (8). The latter effect may be important in vector development for transgenic animals a...

When cloning genomic DNA fragments, the efficiency is greatly reduced as the size of the restriction fragment increases. Libraries derived from the total genome are therefore biased because shorter fragments will be cloned preferentially. When cloning a particular gene, it is most desirable to determine a restriction enzyme that will generate a fragment of a reasonable size for the gene of inte...

gamma-amino butyric acid (gaba) possesses several physiological functions such as neurotransmission, induction of hypotension, diuretic and tranquilizer effects. production of gaba-enriched products by lactic acid bacteria has been a focus of different researches in recent years because of their safety and health-promoting specifities. in this study, glutamate decarboxylase (gad) gene of a loca...

Purpose: A critical factor determining the effectiveness of currently used dendritic cell (DC)–based vaccines is the DC activation or maturation status. We have recently shown that the T-cell stimulatory capacity of DCs pulsed with tumor-antigen–derived peptides can be considerably increased by activating the DCs through electroporation with mRNA encoding CD40 ligand, CD70, and a constitutively...

Hepatitis B Virus (HBV) DNA polymerase transactivated protein 1 (HBVDNAPTP1) is a novel protein transfected by HBV DNA polymerase, which has been screened by a suppression subtractive hybridization technique. In the present study, a yeast two-hybrid system was used to screen the proteins interacting with HBVDNAPTP1 in leukocytes in order to investigate the biological function of HBVDNAPTP1. The...