In this study, novel multifunctional ternary complexes of biotinylated transferrin-avidin-biotin-poly(ethylene glycol)-poly(L-glutamate acid)/poly(2-(2-aminoethylamino) ethyl methacrylate)/doxorubicin-poly(L-aspartic acid)/pDNA (TAB/PIC-D/pDNA complexes) were prepared based on polyion complex micelles (PIC) and the avidin-biotin system, which aimed to target co-delivery of anti-cancer doxorubic...

The conformation of two anti-poly-D-alanyl antibodies was studied before and after interaction with hapten by small-angle X-ray scattering. With both samples a volume contraction could be observed upon interaction with the tetra-D-alanine hapten. The anti-poly-D-alanyl antibodies obtained by immunization with poly-D-alanyl diphtheria toxoid showed a decrease of the volume by 10% and a decrease ...

To test whether the antigen-specific stimulation of low responder-genotype B cells in tetraparental mice is due to a histoincompatibility reaction (allogeneic effect) against these B cells, tetraparental mice were constructed (a) between an Ir-1A low responder to the antigen poly-L(Tyr,Glu)-poly-D,L-Ala--poly-L-Lys. [(T,G)-A--L] and an Ir-1A F1 high responder and (b) between two histoincompatib...

Although some alginate lyases have been isolated from marine bacteria, alginate lyases-excreting bacteria from the Arctic alga have not yet been investigated. Here, the diversity of the bacteria associated with the brown alga Laminaria from the Arctic Ocean was investigated for the first time. Sixty five strains belonging to nine genera were recovered from six Laminaria samples, in which Psychr...

       The purpose of this study was to investigate the effect of additives, poly(ethylene glycol) (PEG) 1450, poloxamer 407, polyvinyl alcohol (PVA) and sodium chloride in order to improve physico-chemical characteristics, encapsulation efficiency and in vitro release of bovine serum albumin, form poly(D,L-lactic-co-glycolic acid) (PLGA) microparticles prepared by the w/o/w solvent evaporation...

polymerase (lane e), and Thermococcus litoralis-derived Vent@ DNA polymerase (lane f) synthesized poly d(A-T), while Pyrococcus sp・-derived PyrobestTM DNA polymerase (lane g) failed to synthesize poly d(A-T). ThoughBcaBESTTM DNA polymerase was known to have RT activity (including Takara's RT-PCR kit), the RT activities of KOD and Vent@ DNA polymerases have not been yet reported. It should be me...

Poly-Ge grown on SiO2 substrates by using one-step ultra-high vacuum chemical vapor deposition (UHVCVD) process with thin Si nucleation layers at very low deposition temperature (350 -C) was demonstrated. The results demonstrated that the polycrystalline silicon (poly-Si) nucleation layer is needed for the growth of polycrystalline germanium (poly-Ge) on SiO2 substrates at low growth temperatur...