the innate immune system utilizes pattern recognition receptors (prrs) to recognize microbes. pathogens contain various molecules with diverse effects on immune response. in this study, we evaluated the effect of dna and protein components derived from two intracellular microorganisms including listeria monocytogenes (l.monocytogenes) and toxoplasma gondii (t. gondii) on dendritic cells (dcs) a...

Background: Baculovirus expression system is one of the most attractive and powerful eukaryotic expression systems for the production of recombinant proteins. The presence of a biomarker is required to monitor transfection efficiency or protein expression levels in insect cells. Methods: The aim of this study was to construct a baculovirus expression vector encoding a copepod super green fluore...

This study was conducted to determine the nutritive value of soybean varieties using in vitro methods and gas production technique.In vitro gas productions and gas production kinetics of soybean variety were determined at 0, 3, 6, 12, 24, 48, 72 and 96 h incubation times. This study revealed that there were variations among five different soybean varieties in terms of chemical...

Background One of the common gonadotropin hormones used for infertility treatment is recombinant Follicle stimulating hormone (rFSH). pH is an environmental factor that can affect production of recombinant proteins with no imposing cost. Culture pH is known as a parameter that significantly affects cell growth and protein production. In this study, tried to investigate the effect of different p...

samples of grass pea seed (lathyrus sativus) were prepared and analyzed for some chemical composition and as well for their anti-nutritional factors. the rumen degradability coefficients and post ruminal digestibility of dry matter and crude protein of unheated vs. oven heated samples were determined, employing in situ and in vitro techniques. results indicated that grass pea seed contained a c...

The aim of this study was to use meta-analysis to identify the enteric methane (CH4) mitigation strategy that reduced CH4 emission without lowering production. To this end, a database initially developed was updated, compiling data from 61 publications (233 experiments) for various observations in dairy cattle on effects of hydrogen sink (H-sink), ionophore, lipid and conc...

The effect of substituting native grass with jengkol (Archidendron jiringa) by-product on fermentation characteristics, rumen microbial profile, methane production, and hydrogen balance using in vitro method was investigated. Seven treatments (different composition of native grass, jengkol peel, jengkol leaves, and concentrate) with five replications in a block randomized desi...

this study was carried out to the determination of nutritive value of canola meal using naylon bag and cumulative gas production techniques in gizel sheep. tow fistulated gizel sheep with average bw 45±2 kg used in a complete randomized design. the cumulative gas production was measured at 2, 4, 6, 8, 12, 16, 24, 36 and 48 h and ruminal dm and cp disappearance were measured up to 96 h. coeffici...

estimation of protein stability is important for many reasons: first providing an understanding of the basic thermodynamics of the process of folding, protein engineering, and protein stability plays important role in biotechnology especially in food and protein drug design. today, proteins are used in many branches, including industrial processes, pharmaceutical industry, and medical fields. a...