Dichloromethane (DCM) is utilized by the strictly anaerobic, acetogenic mixed culture DM as a sole source of carbon and energy for growth. Growth with DCM was linear, and cell suspensions of the culture degraded DCM with a specific activity of 0.47 mkat/kg of protein. A mass balance of 2 mol of chloride and 0.42 mol of acetate per mol of DCM was observed. The dehalogenation reaction showed simi...

1. A gentian-positive strain (a "strain-within-a-strain" variant) has been isolated from a pure culture of a gentian-negative organism. This observation corresponds to that of a "strain-within-a-species" variant, occurring in the enteritidis group, reported some years ago. 2. The Gram reaction and the gentian reaction do not depend, as has been assumed in previous publications, on the specific ...

Analysis of many bacterial genomes is impeded by the inability to separate individual species from complex mixtures of cells or to propagate cells in pure culture. This problem is an obstacle to the study of many bacterial symbionts that live intracellularly in insects and other animals. To recover bacterial DNA from complex samples, we devised a method that facilitates the cloning of DNA fragm...

BACKGROUND The role of viral infections in the etiology of severe community-acquired pneumonia (SCAP) was prospectively evaluated from 2008 to 2012 at a university-level intensive care unit. METHODS Clinical data and microbiological tests were assessed: blood cultures, urine pneumococcal and legionella antigens, Mycoplasma pneumoniae and Chlamydia pneumoniae antibodies from paired serums, and...

root colonization by antagonist bacteria has been considered as a prerequisite for a successful biocontrol. for an effective suppression of plant disease, an efficient and fast colonization is necessary to prevent the establishment of pathogen on the root system. colonization of canola root by strain utpf86 were investigated with and without rhizoctonia solani ag-4, under greenhouse conditions....

Following first recovery of a bacterium by cultural methods there is a period of laboratory manipulation designed to provide a finished product known as a "pure culture." This technical aspect of bacteriology has for its object the production of a bacterial growth, all organisms of which may be thought of as descended from a single parent cell. It is still the belief of many that such a pure cu...

Recent developments in microbial techniques (such as PCR, GE, FISH) have allowed researchers to detect, identify and quantify microorganisms without the limitation of culture-dependent methods. This has given both engineers and scientists a more fundamental understanding about systems containing microorganisms. These techniques can be used to monitor bacteria in wastewater treatment systems, so...

Metacommunity theory proposes that a collection of local communities are linked by dispersal and the resulting compositions are a product of both niche-based (species sorting) and spatial processes. Determining which of these factors is most important in different habitats can provide insight into the regulation of community assembly. To date, the metacommunity organization of heterotrophic soi...