Three different models: the unstructured mechanistic black-box model, the input-output neural network-based model and the externally recurrent neural network model were used to describe the pyruvate production process from glucose and acetate using the genetically modified Escherichia coli YYC202 ldhA::Kan strain. The experimental data were used from the recently described batch and fed-batch e...

Plants under low-oxygen conditions adapt their metabolism by inducing the fermentative pathway, with ethanol as the predominant end product. Activities of pyruvate decarboxylase (PDC) and alcohol dehydrogenase (ADH) are required for this pathway. While a single gene encodes ADH in Arabidopsis, a family of four genes codes PDC. The availability of microarray data sets enabled the relative import...

Two pathways of acetone utilization in the rat have been indicated. One mechatim involves its carboxylation to acetoacetate (1) ; the second, the production of “formate”’ from acetone methyl carbon (2). This formate has been postulated to arise by a 2and l-carbon cleavage in which “acetate”’ is also formed. In the present investigation, the possibility of a direct oxidation of acetone to a 3-ca...

The title compound, C(10)H(11)N(3)O(4), is a condensation product of 4-nitro-phenyl-hydrazine and methyl pyruvate. The complete mol-ecule except for the methyl groups can be considered as a conjugated π system. All non-H atoms are approximately coplanar (r.m.s. deviation 0.117 Å). The crystal packing involves an N-H⋯O hydrogen bond and a π-π inter-action between the aromatic rings, with a centr...

The conversion of octanoate-l-Cl4 to radioacetoacetate and the effect of pyruvate on this process are dependent on the K+ concentration in the incubation medium (1). These results prompted this investigation in which the incorporation of octanoate-l-Cl4 into fatty acids and cholesterol was determined in the presence of non-radioactive additions of pyruvate, succinate, and fumarate. Malonate was...

Histidine-pyruvate aminotransferase and glutamine-phenylpyruvate aminotransferase were purified from rat kidney by the same procedure. The ratio of the two activities remained constant during purification and was unchanged by a variety of treatments of the purified enzyme. Glutamine was found to act as a competitive inhibitor of histidine-pyruvate aminotransferase. These results suggest that ra...

Pyruvate has been shown to decrease the oxidation of palmitic acid by rat liver and kidney slices (l), but to have little influence on octanoate oxidation by liver slices (2, 3). Likewise, prior glucose administration decreased the rate of oxidation of palmitic acid by the rat in viva, but not that of octanoic acid (4). Glucose has also been reported to decrease the oxidation of serum lipides b...

Cytoplasmic thioltransferase purified from rat liver [Axelsson, Eriksson & Mannervik (1978) Biochemistry 17, 2978--2984] catalyses the formation and decomposition of mixed disulphides of proteins and glutathione. The enzyme was found to catalyse the reversible thiol-disulphide interchange between glutathione disulphide and a crude thiol-containing protein fraction from rat liver. This finding i...

Certain phosphocreatine preparations contain a contaminant that inhibits phosphofructokinase and pyruvate kinase assays. The contaminant can be separated from phosphocreatine by anion exchange chromatography. After appropriate purification, phosphocreatine has no effect on phosphofructokinase or pyruvate kinase; thus, there is no evidence that it serves muscle as a regulator of these enzymes. A...