A strategy combining dual-suppression PCR and thermal asymmetric interlaced PCR was used to determine sequences flanking microsatellite regions in Pythium helicoides. The primer pairs were designed to amplify loci containing (AC)n, (GA)n, (AGC)n, (CAC)n(CAA)n, (TCA)n and (CTTT)n repeats from the P. helicoides nuclear genome. The PCR products of each primer pair, amplified from three representat...

Damping-off, root rot and necrotic lesions were observed on sugar beet (Beta vulgaris) seedlings in the Kafrelsheikh Governorate, northern part of Nile River delta, Egypt. Pythium aphanidermatum was identified as causal agent using morphology asexual sexual morphs molecular studies. This is first record P.

One root disease complex often associated with strawberry plants grown in nonfumigated soil is referred to as black root rot. While not currently a widespread problem in properly fumigated commercial production fields, with the impending phase out of the use of methyl bromide and alteration of current fumigation practices this disease complex may once again become a problem. In other strawberry...

pythium irregulare oomycetes adapts with environmental changes including crude oil concentration by changing the composition of lipids in the cytoplasmic membrane and providing the required characteristics for adaptation in improper and stressful environmental situations. it was found that cultivation of pythium irregulare lx oomycetes in the nutrient media with different concentrations of crud...

Corn yield reduction following a cereal rye cover crop has been attributed to, among other factors, allelochemicals released from decomposing residue. The allelopathic effect of 6-methoxy-2-benzoxazolinone (MBOA) was evaluated on corn seedling growth, mycelial growth seven pathogenic species Pythium, and root rot seedlings caused by Pythium spp. at 13, 16, 22 to 23°C (room temperature) using pl...

abstract a total of 270 bacillus strains were isolated from bulk soil and rhizospheric of cucumber in the tonekabon fields (mazandaran province). antifungal activity of bacillus strains was evaluated against pythium aphanidermatum and p. ultimum using dual culture method. 13 strains with more than 10 mm inhibition zone were selected for further study. morphological, physiological, biochemical a...