Supplementary material 1 Real-time quantitative PCR: Sequences of the primers and TaqMan probe for ADAMTS1, 4, 5, 8, 9 and 15 were designed using Primer Express software (Applied Biosystems, Foster City, CA): forward primer 5’-CCATCCCAAGAGTATCACATGTCT-3’, reverse primer 5’-CACTATGACACAGCAATTCTTTTCAC-3’ and TaqMan probe 5’-FAM-CCCACACAAGTCCTGTC-MGB-3 for ADAMTS1; forward primer 5’-TCACTGACTTCCTG...

objectives: this study aimed to compare the microleakage beneath metallic brackets following two different methods of enamel preparation and light curing. materials and methods: a total of 120 bovine deciduous lower incisors were randomly divided into four groups of 30 teeth. the preparations were as follows: group i: acid etching + transbond xt primer + direct illumination, group ii: acid etch...

objectives: micromechanical retention of resin cements to alloys is an important factor affecting the longevity of metal base restorations. this study aimed to compare the bond strength and etching pattern of a newly introduced experimental etchant gel namely nano met etch with those of conventional surface treatment techniques for nickel-chrome (ni-cr) and high noble alloys.  materials and met...

Understanding genetic structure and status of genetic variation of Fasciola hepatica isolates from different hosts, has important implications on epidemiology and effective control of fasciolosis. Random amplified polymorphic DNA (RAPD-PCR) was used to study the genetic variation of F. hepatica in sheep and cattle. DNA was extracted from adult helminthes removed from livers of each infected ani...

En aquest article, Joan Riambau, director literari de tres segells Penguin Random House, exposa, primer tot, l’estat l’edició en català relació amb la bona acollida que hi han tingut els best-sellers traduïts del castellà, i, a continuació, Francesc Parcerisas l’entrevista sobre determinades particularitats món editorial català.

The purpose of this study was to evaluate the effects of acidic primers on adhesive bonding to sintered aluminum oxide (alumina). Alumina disks were primed with one of the following materials: Acryl Bond, All Bond 2 Primer B, Alloy Primer, Estenia Opaque Primer, Eye Sight Opaque Primer, M.L. Primer, MR. Bond, and Super-Bond Liquid. Specimens were then bonded with an acrylic resin initiated with...

Aptamers, or single stranded oligonucleotides, are produced by systematic evolution of ligands by exponential enrichment, abbreviated as SELEX. In the amplification and regeneration step of SELEX technique, dsDNA is conversed to ssDNA. Asymmetric PCR is one of the methods used for the generation of ssDNA. The purpose of this study was to design a random DNA library for selection of aptamers wit...

The results could be summarized as follow: 1Primer OP-B01 gave 7 monomorphic and 13 polymorphic fragments (65%). 2The Primer OP-B02 and Primer OP-B05 gave 4 monomorphic fragments with 14 polymorphic fragments (79%). 3The Primer HA-98 gave 4 monomorphic fragments with 19 detected polymorphic 83%. 4The Primer HA-99 and HB-12 gave 3 monomorphic fragments and 17 polymorphic 85 and 86%, respectively...

To provide feasible primer sets for performing a polymerase chain reaction (PCR) experiment, many primer design methods have been proposed. However, the majority of these methods require a relatively long time to obtain an optimal solution since large quantities of template DNA need to be analyzed. Furthermore, the designed primer sets usually do not provide a specific PCR product size. In rece...