OBJECTIVES A study was conducted in coke oven workers to evaluate the biological consequences of the exposure of these workers, particularly production of potential genotoxic factors. METHODS 60 coke oven workers and 40 controls were recruited in the same iron and steel works. Exposure to polycyclic aromatic hydrocarbons (PAHs) was assessed by job and measurement of 1-hydroxypyrene (1OHP) in ...

The mechanism of glucocorticoid-induced internucleosomal DNA cleavage and cytolysis of lymphatic cells is not known. Recent data (Compton, M.M., and Cidlowski, J.A. (1987) J. Biol. Chem. 262, 8288-8292) suggested that in vivo treatment of rat thymocytes with glucocorticoids induces a nucleolytic "lysis gene" product(s) responsible for lymphocytolysis. In this paper, the possibility that lymphoc...

RNA from a rat liver tumor (Morris hepatoma 5123tc) was used to construct cDNAs together comprising the complete coding sequence of rat oncomodulin mRNA. Information obtained from these cDNAs as well as from primer extension analysis gave a deduced length for the complete oncomodulin mRNA of approximately 680 nucleotides (excluding the poly(A) tail) including a 5'-untranslated region of 97 +/- ...

An altered expression of the Yc subunit gene of rat glutathione S-transferase (GST) in the liver of the LEC rat, which is a mutant strain with spontaneous hereditary hepatitis associated with severe jaundice, has been reported. To provide further information concerning the structure of the Yc subunit gene, we carried out the Southern blot hybridization analysis of DNA samples from rats of eight...

Hormone responsiveness of autonomous and diversely hormone-responsive mammary tumors of the rat was assayed in organ cultures by measuring DNA synthesis. Rat sera rich in hormones secreted by mammotropic pituitary cells were compared with sera of normal female and male and of ovariectomized and hypophysectomized rats. Mammotropic sera invariably stimulated DNA synthesis of hormoneresponsive tum...

Isolated rat liver cells have been used to measure DNA repair synthesis induced by ultraviolet light and aflatoxin. Age, sex, and diet of the rats were found to influence DNA repair in rat liver cells, as measured by [3H]thymidine uptake by the cells. These effects were found to be due to variations in the thymidine pool size and should serve to indicate that high-specific-activity thymidine (i...

Purified preparations of the parvovirus, Kilham rat virus, have associated with them a protein with DNA polymerase activity. The enzyme has been separated from the other two or three viral proteins and purified 63-fold. The viral associated enzyme was found in a single peak of DNA polymerase activity after chromatography on DEAE-cellulose, DNA-cellulose, and phosphocellulose columns. It shares ...

Experiments by Bendich et al. (1, 2) and by Polli and Shooter (3, 4) have suggested that the deoxyribonucleic acids might be tissue specific. Bendich et al. (1) found that the DNA of rat spleen, kidney, brain, and intestine could be distinguished by their chromatographic profiles on diethylaminoethyl-cellulose. The chromatographic differences between rat kidney and brain DNA were particularly p...