I N 1948, Laki and Lorand1 observed that “fibrin clots” formed by the addition of calcium throm in to purified fibrinogen solutions were readily soluble in 5 NI urea and 1 per cent monochloroacetic acid. “Plasma clots” formed Iw the recalcification of oxalated plasma were insoluble in these solvents. Urea insolube clots could also be prepared 1w the addition of small amounts of plasma to the pu...

The coagulation cofactor Va (FVa) is a noncovalent heterodimer consisting of a heavy chain (FVaH) and a light chain (FVaL). Previously, the fibrinolytic effector plasmin (Pn) has been shown to inhibit FVa function. To understand this mechanism, the fragmentation profile of human FVa by Pn and the noncovalent association of the derived fragments were determined in the presence of Ca(2+) using an...

Recent interest in the treatment of disseminated intravascular coagulation with heparin (1, 2) has caused some clinicians and laboratory workers to express doubt concerning the accuracy of fibrinogen methods based on thrombin-induced fibrin clot formation and subsequent measurement of the fibrin by biuret methods. It was reasoned that inaccurately low results would be produced owing to the pres...

The activity of intact cells or of extracts from cells may be studied in blood coagulation systems to elucidate the problems of coagulation or to investigate the properties of cells and their extracts. The present report is concerned primarily with the latter objective. Since the beginning of this century aqueous extracts of cells have been known to accelerate clotting. In 1916 McLean prepared ...

Objective: Search of best treatment plan for non-small lung cancer (LC) patients (LCP) was realized. Methods: In trial (1985-2008) the data of consecutive 535 LCP after complete resections (R0) (age = 57.3 ± 8.2 years; male-482, female-53; tumor diameter: D = 4.7 ± 2.2 cm; pneumonectomies-222, lobectomies-313, combined procedures with resection of pericardium, atrium, aorta, VCS, carina, diaphr...