This paper describes the use of Au nanoparticle (NP)-containing hydrogel microstructures in the development of electrochemical enzyme-based biosensors. To fabricate biosensors, AuNPs were conjugated with glucose oxidase (GOX) or horseradish peroxidase (HRP) molecules and were dispersed in the prepolymer solution of poly(ethylene glycol) diacrylate (PEG-DA). Vinylferrocene (VF) was also added in...

Microfabricated surfaces have been widely utilized for defining adhesion of single cells or groups of cells of various kinds. Beyond simple control of cell attachment, it is often important to monitor the molecules released by cells. Co-immobilizing miniature sensors alongside cells enables more sensitive detection of secreted factors and may allow for such detection to happen within the contex...

In the present study, a temperature-sensitive gel composed of chitosan, carboxymethyl chitosan and glycerophosphate was prepared and loaded with chitosan microspheres encapsulating levofloxacin. The bioavailability of levofloxacin and the safety of this novel opthalmic drug delivery formulation were evaluated. Levofloxacin chitosan microspheres were prepared using the ionic gelation method, and...

A new way is demonstrated to develop a bio-inspired coacervate hydrogel by following catechol chemistry showing injectable and re-moldable physical properties. The formed coacervate shows potential long-term stability under water. Depending on pH, formation of the coacervate has been verified which is confirmed by XPS and zeta potential measurements.

conclusions this study showed that compared to standard drug treatment, treatment with hydrogel containing peptide had substantial antibacterial effects on s. aureus wound infections in mice. results the highest and lowest numbers of wounds were observed on day 2 in the negative and positive control groups, respectively. during the 8-day period, the positive control and hydrogel containing pept...

cell-based biosensors have become a research hotspot in biosensors and bioelectronics fields. the main feature of cell-based biosensors is the immobilization of living cell on the surface of transducers. different types of polymers which are used as scaffolds for cell growth should be biocompatible and have reactive functional groups for further attachment of biomolecules. in this work, the cel...

Polyacrylic acid/ octavinyl polyhedral oligomeric silsesquioxane, nanocomposite hydrogel with 3-D network was synthesized via radical polymerization. Octavinyl polyhedral oligomeric silsesquioxane was used as crosslinker and nanofiller simultaneously in the preparation of the hydrogel. Hydrogel adsorption performance was determined by adsorption of methylene blue. The adsorption capacity was ev...

PURPOSE To devise a procedure for direct estimation of corneal oxygen consumption in human subjects. METHODS Tear oxygen tension (PO2) was measured at the posterior surface of two standard hydrogel contact lenses (38% water, 0.2 and 0.06 mm thick, oxygen transmissibility [Dk/t] = 4.2 and 14 x 10(-9) cm x mL O2/mL x sec x torr) and one newly available hydrogel-silicone polymer lens (Dk/t = 99 ...

Hydrogels are the 3 dimensional cross linked polymeric networks which absorb the water 10-20 times of its molecular weight. Hydrogels are sensitive to different environmental stimuli like pH, temperature, ion, electric signals, light, pressure, antigen specific, enzyme sensitive, and glucose levels. When the environment is get change they get swell, and swelling is the most important characteri...

Wharton’s jelly mesenchymal stem cells (HWJMSCs) hold promise for myocardial regeneration, but optimal treatment regimen (preferably with a growth factor) is required to maximize functional benefits. The aim of this study was to explore the cardioprotective and angiogenesis effects of HWJMSCs combined with insulin-like growth factor-1 (IGF-1) in the treatment of acute myocardial infarction. Th...