In vitro regeneration of plants from root cultures of Swertia chirata Buch.-Ham. ex Wall was obtained. Root explants from axenic shoot cultures were used for shoot induction. Optimal shoot regeneration without any callus intervention was observed on 1/2 MS (Murashige and Skoog’s medium, 1962) + 4.44 μM 6, benzylaminopurine (BAP) +1.07 μM α-naphthalene acetic acid (NAA).Regenerated shoots were f...

Rapid in vitro multiplication of shoots has been described using capitular sections and leaf explants in Gerbera jamesonii. Three types of basal media regimes were used including MS and two of its modified forms⎯MSI and MSA. Shoot induction was achieved on all three regimes but MSI medium supplemented with Kn (4 mg/l) and IAA (0.5 mg/l) was ideal for shoot bud initiation (8-11) from capitular s...

the jatropha curcas l., a plant belongs to euphorbiaceae family, which has great economic and medicinally value. there is high amount of oil in seeds of this plant. because of low germination ability of seed, propagation of jatropha curcas via seed is not usual. the purpose of this work is optimizing of jatropha curcas tissue culture for optimization of callus formation and propagation through ...

Plant propagation originated  from the mature tissues is the most desirable method in producing clonal plants, however microbial contamination usually is the main concern during regeneration processes. Moreover, commercial production of plants using clonal propagation is considered as high throughput method due to the   removal of seasonal barriers, lower cost of production with higher yield wh...

Background In vitro organogenesis is one of the key techniques associated with genetic transformation, as it determines the successful regeneration of transgenic plants after co-cultivation with bacteria. Therefore, the development of efficient regeneration protocols is the most critical step in developing genetic transformation. Protocols have been developed for several species of eucalyptus. ...

Rhizome explants were used to develop a protocol for in vitro plantlet regeneration of Podophyllum hexandrum, a critically endangered medicinal plant, through direct organogenesis. Highest rate of multiple shoot formation was noted in MS medium supplemented with 11.42 μM IAA within three months. A synergistic effect of 2.68 μM NAA and 11.1 μM BAP was second best. The 1⁄2 MS liquid medium with 1...