The glutathione S-transferases from the porina moth, Wiseanna cervinata, were purified by affinity chromatography, cation-exchange chromatography and preparative isoelectrofocusing. The major transferase (IV) was purified to homogeneity by a factor of 530-fold with a yield of 83%. Other transferases present were purified to a smaller degree (approx. 50-fold) to a stage of near-homogeneity. The ...

The human blood groups (ABO) are known to be determined by the terminal carbohydrate residues attached to common carbohydrate chains of the red cell surface. Specific transferases, i.e., N-acetylgalactosaminyltransferase (A enzyme) in blood group A persons and galactosyltransferase (B enzyme) in blood group B persons. are responsible for transferring the carbohydrates to the terminal galactose ...

Since their discovery, bacterial glutathione (GSH)transferases have been characterized in terms of their ability to catalyse a variety of different reactions on a large set of toxic molecules of xenobiotic or endobiotic origin. Furthermore the contribution of different residues in the GSH-binding site to GSH activation has been extensively investigated. Little is known, however, about the contr...

Indomethacin inhibited rat liver glutathione S-transferases (EC 2.5.1.18). Its inhibition was non-competitive with respect to 3,4-dichloronitrobenzene with an apparent Ki of 5.3 X 10(-5) M and uncompetitive with respect to glutathione with an apparent Ki of 4.0 X 10(-5) M. 4-Chlorobenzoic acid and 5-methoxy-2-methylindole-3-acetic acid, two metabolites of indomethacin, were weak inhibitors of t...

Glutathione S-transferases (GSTs) play an important role in detoxification of various toxic compounds like carcinogens in cigarette smoke and tobacco by conjugating to toxic compounds and inactivating their hazardous effect. Variation in Glutathione S-Transferases (GSTs) genes may alter the catalytic efficiency of GST isoenzymes leading to potential increase in cancer susceptibility due to vari...

The activity of initiation factors obtained from host livers and transplantable H5123 hepatomas of Buffalo rats was investigated using an in vitro assay of protein synthesis. Initiation factors obtained by washing ribosomes with l M KC1, KCl-washed ribosomes, 60 S and 40 S ribosomal subunits, and transferases were prepared from liver and from tumor. The activity in polyuridylic acid [poly(U)]-d...