Human liver glutathione S-transferases (GSH S-transferases) were fractionated into cationic and anionic proteins. During fractionation with (NH4)2SO4 the anionic GSH S-transferases are concentrated in the 65%-saturated-(NH4)2SO4 fraction, whereas the cationic GSH S-transferases separate in the 80%-saturated-(NH4)2SO4 fraction. From the 65%-saturated-(NH4)2SO4 fraction two new anionic GSH S-tran...

paracetamol (acetaminophen, pcm) is a widely used over-the-counter analgesic and antipyretic drug. intake of a large dose of pcm may result in severe hepatic necrosis. oxidative stress is mediated by oxidative capacities of the pcm metabolite (n-acetyl-para-benzo quinoneimine, napqi), which covalently binds to proteins and other macromolecules to cause cellular damage. at low doses, napqi is co...

glutathione s-transferase (gst) is a superfamily enzyme which plays a major role indetoxification of xenobiotic compounds by catalyzing the conjugation of xenobiotic to cellular glutathione(gsh). gst-p is an important class of gsts which is expressed during the early stage of life and duringdevelopmental stages. its activity is relatively high during embryogenesis and immediately after birth an...

The impact of PGF2? on total proteins, free amino acids and the amino transferases in the gonads of silkworm, Bombyx mori L. were investigated in the present experiment. The effect of PGF2? resulted in increase in the total protein and free amino acid contents in the gonads of silkworm larvae indicating improved structural and dynamic levels of organization. The enhanced activity levels of amin...

The N-end rule relates the in vivo half-life of a protein to the identity of its N-terminal residue. Primary destabilizing N-terminal residues (Nd(p)) are recognized directly by the targeting machinery. The recognition of secondary destabilizing N-terminal residues (Nd(s)) is preceded by conjugation of an Nd(p) residue to Nd(s) of a polypeptide substrate. In eukaryotes, ATE1-encoded arginyl-tra...

A purification scheme was devised that resulted in the resolution of a number of basic glutathione S-transferases from rat liver, three of which contained two subunits of molecular mass 23500 Da (i.e. Yb monomers). These were identified as transferases D, C and A by their elution positions from CM-cellulose and their specific activities towards a variety of substrates. Hybridization, immunotitr...

Paracetamol (acetaminophen, PCM) is a widely used over-the-counter analgesic and antipyretic drug. Intake of a large dose of PCM may result in severe hepatic necrosis. Oxidative stress is mediated by oxidative capacities of the PCM metabolite (N-acetyl-para-benzo quinoneimine, NAPQI), which covalently binds to proteins and other macromolecules to cause cellular damage. At low doses, NAPQI is ...

the impact of pgf2? on total proteins, free amino acids and the amino transferases in the gonads of silkworm, bombyx mori l. were investigated in the present experiment. the effect of pgf2? resulted in increase in the total protein and free amino acid contents in the gonads of silkworm larvae indicating improved structural and dynamic levels of organization. the enhanced activity levels of amin...