Several analytical techniques based upon the use of substrate-polyacrylamide gel electrophoresis were evaluated to achieve characterization of aspartate proteases in fish stomach. Since aspartate proteases of fish are more stable at high pH than mammalian pepsins, the most accurate technique for activity assessment is electrophoresis at neutral pH and revealing of such activity at low pH with h...

S. aureus is a pathogenic bacterium that requires iron to carry out vital metabolic functions and cause disease. The most abundant reservoir of iron inside the human host is heme, which is the cofactor of hemoglobin. To acquire iron from hemoglobin, S. aureus utilizes an elaborate system known as the iron-regulated surface determinant (Isd) system. Components of the Isd system first bind host h...

This article presents a simple and efficient measurement system for quantitative sensing of blood hemoglobin (Hgb) using curcumin nanoparticles (CURNs). The Hgb monitoring is based on CURNs aggregation in the presence of Hgb, which leads to a decrease in the absorption intensity of CURNs. In this study the analytical signal for the measurement of blood Hgb concentration was defined as the diffe...

patients and methods in this cross-sectional study, the hemoglobin levels of 52 patients with end-stage renal disease were measured before and after hemodialysis. the required erythropoietin doses and the differences in cost were calculated based on the hemoglobin levels before and after hemodialysis. a model to predict the adjusted erythropoietin dosages based on post-hemodialysis hemoglobin l...

Cellulase production by the fungus Trichoderma reesei was studied using sugar beet pulp (SBP) as a substrate. The subculture medium was a salt solution consisting of KH2PO4, CaCl2, etc. Fungal cells were sub-cultured in an orbital shaker (180 rpm) at 30°C for 1-2 generations (two days for each generation) and were then used as an inoculum. Exponential cells were inoculated into a medium contain...