نتایج جستجو برای: استخراج igy

تعداد نتایج: 43756  

2016
Chi-Hsin Lee Yu-Ching Lee Sy-Jye Leu Liang-Tzung Lin Jen-Ron Chiang Wei-Jane Hsu Yi-Yuan Yang

The venom of the banded krait (Bungarus multicinctus), one of the major venomous species in Taiwan, contains neurotoxic venom proteins (B. multicinctus proteins) that pose a serious medical problem in tropical and subtropical countries. Even though horse-derived serum is an efficient therapy against snake venom, it is associated with a high cost and side effects. Therefore, developing a more co...

ژورنال: :مجله دانشگاه علوم پزشکی بابل 0
مهدی پورامیر m pour amir دانشگاه علوم پزشکی بابل، گروه بیوشیمی-بیوفیزیک، تلفن: 5-2229591-0111 محمدجواد رسائی mj rasaei

سابقه و هدف: خالص سازی ایمونوگلوبولین y از زرده تخم مرغ با روش های کروماتوگرافی از تحقیقات جدید می باشد و هدف این تحقیق نیز دستیابی به روش ساده و ارزان برای خالص سازی igy از زرده تخم مرغ می باشد. مواد و روش ها: زرده تخم مرغ بعد از عبور از مش نایلونی در داخل استوانه مدرج جمع آوری شد. زرده با 10 برابر حجم hcl (برابر با 3mm) رقیق شده و سوسپانسیون سانتریفوژ گردید. سولفات آمونیوم تا 60% اشباع اضافه ...

پورامیر, مهدی, رسائی, محمدجواد ,

سابقه و هدف: خالص سازی ایمونوگلوبولین Y از زرده تخم مرغ با روش های کروماتوگرافی از تحقیقات جدید می باشد و هدف این تحقیق نیز دستیابی به روش ساده و ارزان برای خالص سازی IgY از زرده تخم مرغ می باشد. مواد و روش ها: زرده تخم مرغ بعد از عبور از مش نایلونی در داخل استوانه مدرج جمع آوری شد. زرده با 10 برابر حجم HCL (برابر با 3mm) رقیق شده و سوسپانسیون سانتریفوژ گردید. سولفات آمونیوم تا 60% اشباع اضافه ...

Journal: :iranian journal of allergy, asthma and immunology 0
m. pour-amir m.j. rasaee qujeq giir. asadikaram m. frozeh moqhadam

the use of immunoglobulin y (igy) instead of polyclonal antibodies prepared in mammals has been reported recently. these antibodies are found to have better properties in terms of specificity and ease of large scale production. in this study, proteins from egg yolk were extracted with acid and chloroform. the result of cellulose acetate electrophoresis at ph 9 showed some extra bands which were...

Journal: :ALTEX 2003
Esteban Gutiérrez Calzado Eduardo Cruz Mario Tomás Samón Chávez Ernesto Vázquez Luna Zelma Corona Ochoa Rüdiger Schade

During the last ten years the extraction of specific antibodies (ab) from the yolk of eggs of immunised chickens is more and more accepted as an useful alternative to the immunisation of mammals. The subject of this work is the immunisation of chickens with human IgG and the extraction of specific anti human IgG ab from egg yolk in order to obtain monospecific Coombs reagent. 12 Leghorn hens (2...

Journal: :archives of clinical infectious diseases 0
hossein basiri department of microbiology, islamic azad university, arak branch, arak, ir iran seyed latif mousavi department of biology, shahed university, tehran, ir iran; department of biology, faculty of science, shahed university, tehran-qom freeway, opposite imam khomeini's shrine, tehran, iran. iraj rasooli department of biology, shahed university, tehran, ir iran mohsen basiri department of biology, shahed university, tehran, ir iran davood yadegarynia infectious diseases and tropical medicine research center, shahid beheshti university of medical sciences, tehran, ir iran

background helicobacter pylori multiplies and causes infection in human gastric mucosal layer. new approaches have focused on using specific treatments, such as immunotherapy, to limit this infection. urease, as one of the most important virulent and antigenic factors of the bacterium, is a suitable target for this purpose. patients and methods in order to prepare recombinant proteins, the synt...

Journal: :Avian diseases 2009
Carol A Fassbinder-Orth Erik K Hofmeister Carolyn Weeks-Levy William H Karasov

West Nile virus (WNV) causes morbidity and mortality in humans, horses, and in more than 315 bird species in North America. Currently approved WNV vaccines are designed for parenteral administration and, as yet, no effectiveoral WNV vaccines have been developed. WNV envelope (E) protein is a highly antigenic protein that elicits the majority ofvirus-neutralizing antibodies during a WNV immune r...

Journal: :Applied and environmental microbiology 2016
Chi-Hsin Lee Yu-Ching Lee Meng-Huei Liang Sy-Jye Leu Liang-Tzung Lin Jen-Ron Chiang Yi-Yuan Yang

Snake venom protein from Deinagkistrodon acutus (DA protein), one of the major venomous species in Taiwan, causes hemorrhagic symptoms that can lead to death. Although horse-derived antivenin is a major treatment, relatively strong and detrimental side effects are seen occasionally. In our study, yolk immunoglobulin (IgY) was purified from eggs, and DA protein was recognized using Western blott...

Journal: :Physiological and biochemical zoology : PBZ 2011
Sophie Bourgeon Sarah Guindre-Parker Tony D Williams

We investigated the effects of ecological context (by comparing data from two consecutive years) and experimentally manipulated nestling developmental conditions (large vs. small brood size) on immune function (immunoglobulin Y [IgY]) and oxidative stress in nestling European starlings Sturnus vulgaris. On the basis of annual differences in chicks' morphological traits and body masses close to ...

Journal: :Veterinary parasitology 2009
S H Lee H S Lillehoj D W Park S I Jang A Morales D García E Lucio R Larios G Victoria D Marrufo Erik P Lillehoj

Avian coccidiosis is caused by several distinct species of Eimeria protozoa and is the major parasitic disease of poultry of economic importance. As an alternative strategy to control avian coccidiosis without using prophylactic medications, we have investigated the efficacy of inducing passive immunity against coccidiosis by orally feeding hyperimmune IgY antibodies. In this study, a commercia...

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