A bacterial strain C5 that can produce new type of marine esterase was isolated and screened from marine sludge. According to 16S rRNA sequence analysis and physiological and biochemical experiments, the strain was identified as Bacillus subtilis. A single isozyme with a molecular weight of 86 kDa was observed by SDS-PAGE and native-PAGE. On this basis, the mechanism of esterase B1 secreted by ...

A possible breakthrough in the treatment of diabetes was made with the discovery that a fungal natural product, demethylasterriquinone B1 (DAQ B1), is an orally active, small-molecule mimic of insulin. Subsequent work has shown that the glucose-lowering effects of DAQ B1 are not accompanied by enhanced vascular proliferation, which is a side effect of chronic insulin administration that can lea...

The biogenesis of ribosomes in vivo is an essential process for cellular functions. Transcription of ribosomal RNA (rRNA) genes is the rate-limiting step in ribosome biogenesis controlled by environmental conditions. Here, we investigated the role of folate antagonist on changes of DNA double-strand breaks (DSBs) landscape in mouse embryonic stem cells. A significant DSB enhancement was detecte...

background and objectives: the development of molecular biological techniques provide a method for direct detection and identification of pathogens. this study attempted to compare bacterial culture and pcr to detect s. agalactiae in cattle milk samples. the pcr method was based on using primers v1 and v2 derived from the 16s rrna gene. materials and methods: one hundred milk samples were colle...

In most Japanese populations of dojo loach (Misgurnus anguillicaudatus), gonochoristic diploids genetically diversified groups (A and B, further subdivided into B1 B2) are present, whereas unisexual clonal lineages inhabit certain localities in the Hokkaido Ishikawa Prefectures Japan. Through a series genetic studies including DNA markers, loaches were deemed to originate from hybridization eve...

To the Editor: In a recent issue of The Journal of Experimental Medicine, Griffin and Rothstein described two subsets within the human B1 cell compartment: a major CD11b  subset (9/10th of the population) and a minor CD11b + subset (1/10th of the population), with the latter being increased in lupus (Griffin and Rothstein, 2011). Griffin and Rothstein (2011) analyzed these two B1 cell subsets ...

Cyclin genes and their products are important regulatory participants in the eukaryotic cell cycle. It is well established that cyclin B1 protein forms a complex with cyclin-dependent kinase 1 (CDK1), which, when activated, initiates mitosis. We have previously established that cyclin B1 gene expression is posttranscriptionally regulated in regenerating rat liver after 70% partial hepatectomy (...

In eukaryotic cells, ribosomal RNA (rRNA) gene is transcribed by RNA polymerase I (pol I) in the nucleolus as a large (40 S-47 S) precursor RNA (pre-rRNA) (for reviews, see refs. [1,2]). The mature rRNA species (28 S, 18 S and 5.8 S) are formed after a series of specific endonucleolytic cleavage reactions (for a review, see ref. [3]). The rRNA gene (rDNA) is highly reiterated and is arranged in...

A pentapeptide open reading frame equipped with a canonical ribosome-binding site is present in the Escherichia coli 23S rRNA. Overexpression of 23S rRNA fragments containing the mini-gene renders cells resistant to the ribosome-inhibiting antibiotic erythromycin. Mutations that change either the initiator or stop codons of the peptide mini-gene result in the loss of erythromycin resistance. No...

Considering the importance of Theileria equi infection in horse breeding industry and marketing, in the present study, we aimed to determine the prevalence of T. equi among dromedaries in central Iran, where a considerable number of camels and horses are raised and equine theileriosis is quite prevalent. For this purpose, a total of 161 blood samples from camels were examined in terms of T. equ...