Fatty acid (FA) composition of suet oil (SO) was measured by precolumn methylesterification (PME) optimized using a Box-Behnken design (BBD) and gas chromatography/electron ionization-quadrupole mass spectrometry (GC-EI-qMS). A spectral library (NIST 08) and standard compounds were used to identify FAs in SO representing 90.89% of the total peak area. The ten most abundant FAs were derivatized ...

A fast and efficient method based on a polyethylene glycol (PEG) 600/(NH₄)₂SO₄ aqueous two-phase system for extracting lectin from Zihua snap-bean (Phaseolus vulgaris) seeds was established. According to a Box-Behnken design (BBD), involving four factors at three levels each subjected to analysis of variance (ANOVA) and response surface analysis, the protein recovery and the purification factor...

Flexirubins are the unique type of bacterial pigments produced by the bacteria from the genus Chryseobacterium, which are used in the treatment of chronic skin disease, eczema etc. and may serve as a chemotaxonomic marker. Chryseobacterium artocarpi CECT 8497, an yellowish-orange pigment producing strain was investigated for maximum production of pigment by optimizing medium composition employi...

BACKGROUND This study performed optimized extraction, preliminary characterization, and in vitro antioxidant activities of polysaccharides from Glycyrrhiza uralensis Fisch. MATERIAL AND METHODS Three parameters (extraction temperature, ratio of water to raw material, and extraction time) were optimized for yields of G. uralensis polysaccharides (GUP) using response surface methodology with Box-...

Streptomyces thermoviolaceus NT1, an endophytic isolate, was studied for optimization of granaticinic acid production. It is an antimicrobial metabolite active against even drug resistant bacteria. Different media, optimum glucose concentration, initial media pH, incubation temperature, incubation period, and inoculum size were among the selected parameters optimized in the one-variable-at-a-ti...

In this study, the primary paper-mill sludge characterized as containing 51% glucan was used to optimize enzymatic saccharification process for production of bioethanol using a Box–Behnken design (BBD). Polyethylene glycol 4000 (PEG-4000) surfactant-assisted dried (DPS) showed 12.8% improvement in efficiency. There statistically significant effect solid enzyme loading and time on DPS at 95% con...

In this study, a Box-Behnken Design (BBD) has been used to optimize the recovery of bioactive compounds from jabuticaba (Myrciaria cauliflora) by-products through microwave-assisted extraction (MAE). Thus, experimental design was followed by response surface methodology (RSM) that would allow investigating influence four independent variables (temperature, solvent composition, pH, and sample-so...

Objective: The present study aimed to develop and characterize Chitosan coated Alginate Nanocapsules loaded with M-Insulin Concanavalin A Complex for glucose-responsive delivery. Methods: Preformulation studies were performed on the Insulin human recombinant prepared by ionic gelation method chitosan using electrostatic attraction. formulation variables optimized Box-Behnken design (BBD) help o...

Nowadays, oily wastewater is increasing along with the growth of various industries. So, wastewater treatment is necessary in order to protect the environment. In this study, a mullite ceramic membrane was prepared. Then, oily wastewater treatment with 200 mg L-1 concentration was investigated by the response surface methodology based on Box-Behnken design (BBD) using Design-Expert 7...

An efficient method was employed for the ash and pyrite sulphur removal from bitumen using column flotation process. The bitumen samples containing 9.6% sulphur (6.81% in the pyrite sulphur form) and 26.4% ash were successfully showed up to 75.20% of pyrite sulphur (e.g.; 51.66% of total sulphur) and 70.81% of ash removal. The Box-Behnken Design (BBD) was employed to design the experiments, to ...