Micropropagation of Vaccinium spp. using Murashige Skoog (MS) and benzylaminopurine (BAP) has been successfully developed. This work aimed to determine the effect different types substrates auxins (naftalen acetic acid NAA, indol IAA y, butyric IBA) on ex vitro establishment rooting nodal segments V. corymbosum Var. Biloxi create a donor bank blueberry plants. The influence five three concentra...

Licorice plants, Glycyrrhiza glabra, G. uralensis, and G. inflata, were investigated for callus induction using Murashige and Skoog (MS) medium combined with auxins and cytokinins. After 4 weeks of culture, 33-100% of leaf or stem explants formed calli. Maximum of shoot induction from callus cultures was achieved by G. inflata stem explants cultured on MS medium supplemented with 1 mg/l alpha-n...

BACKGROUND AND AIMS Ilex khasiana is a rare and critically endangered holly endemic to the Khasi Hills of Meghalaya, India, and confined to a small number of pocket areas. In addition to conventional methods of propagation, endemic and threatened plants such as this could be more effectively multiplied and conserved using in vitro methods. Such techniques have the additional advantage of having...

The influence of explant nodal positions on the in vitro shoot growth and proliferation were studied in the two rose cvs. ‘Bianca’ and ‘El Torro’. Third, fourth and fifth nodal explants were cultured on the modified MS medium supplemented with 1.0 and 5.0 mg/l BA. In both the cultivars, higher rate of proliferation (‘Bianca’ 3.75; ‘El Torro’ 2.65) were obtained from the explants distal to the a...

sugar type and growth regulators are essential fundamental components of tissue culture media. in this study, different concentrations of some carbohydrate and growth regulators during anther culture and producing somatic embryo of datura stramonium l. was investigated in ms medium. the ms medium supplemented with 0.5 mgl-1 kinetin and 2 mgl-1 2,4-d, and also the medium supplemented with 0.25 m...

ABSTRACT Lavender (Lavandula dentata) has several medicinal applications, both in the fresh form and after industrial processing. Productivity increase could be obtained through mass propagation, expanding possibility of obtaining raw material for production essential oil. The objective this work was to develop a micropropagation protocol from explants seeds. Aspects vitro germination, multipli...

We developed a protocol for the production of shoots from bud explants from 1- to 7-year-old trees of western white pine (Pinus monticola Dougl.). The best explant was a 2-mm-thick cross-sectional slice of the early winter bud. Genotype of the donor tree was a significant factor affecting shoot production, but more than 80% of the genotypes tested produced shoots. Of the media tested, bud slice...

An efficient plant regeneration protocol has been developed from leaf explants of Stevia rebaudiana, an economically and medicinally important herbaceous plant species belonging to the family Asteraceae. Plant regeneration and multiple shoot proliferation was initiated from leaf explants cultured on MS medium supplemented with various concentrations of 6-benzyladenine (BA) and kinetin (KN) in c...

A procedure for rapid clonal propagation of Passiflora edulis Sims. f. flavicarpa Deg. (Passifloraceae) has been developed in this study. Nodal explants were sterilized with 0.1% HgCl2 and inoculated on Murashige and Skoog (MS) basal medium. The addition of 2.0 mgL(-1) 6-benzylaminopurine (BAP) to MS medium caused an extensive proliferation of multiple shoots (8.21 ± 1.13) primordial from the n...