نتایج جستجو برای: bovine embryonic development
تعداد نتایج: 1523999 فیلتر نتایج به سال:
Despite the application of numerous supplements to improve in vitro culture (IVC) conditions of mammalian cells, studies regarding the effect of fetal bovine serum (FBS) on mammalian early embryogenesis, particularly in relation to redox homeostasis, are lacking. Herein, we demonstrated that early development of in vitro-produced (IVP) porcine embryos highly depends on the combination of FBS su...
The level of muscle development in livestock directly affects the production efficiency livestock, and contents intramuscular fat (IMF) is an important factor that meat quality. However, molecular mechanisms through which circular RNA (circRNA) IMF remains largely unknown. In this study, we isolated myoblasts preadipocytes from fetal bovine skeletal muscle. Oil Red O BODIPY staining were used t...
Early embryonic development may be affected by adrenal hyperactivity in stressful situations which lead to endocrine changes the embryo environment. A sensitive period porcine is 4-cell stage when genome activation occurs. mixed vivo-in vitro system was implemented test whether an altered milieu around this could affect and blastocyst quality model. After maturation fertilisation, presumptive z...
Transcription factor TEA domain family transcription factor 4 (Tead4) is one of the key factors involved in the differentiation of the trophectoderm (TE) in murine embryos. However, knowledge on the roles of TEAD4 in preimplantation development during bovine embryos is currently limited. This study examined the transcript and protein expression patterns of TEAD4 and attempted to elucidate the f...
The objective of this study was to assess whether the exposure of zona pellucida-intact bovine embryos to the proteolytic enzyme, trypsin, during embryo washing has a detrimental effect on their subsequent survival and development. Embryos were collected nonsurgically from superovulated cows (n = 19) 7.5 d after insemination. Grade 1 and Grade 2 embryos were washed 12 times in modified Dulbecco...
Pituitary growth hormone (GH) stimulates postnatal growth and metabolism. The role of GH and its receptor (GHR) during prenatal development, however, is still controversial. As shown by reverse transcription polymerase chain reaction (RT-PCR), bovine in vitro fertilization embryos synthesized the transcript of GHR from Day 2 of embryonic life onwards. Real time RT-PCR revealed that synthesis of...
High environmental temperatures observed during the hot months of the year reduce fertility in lactating dairy cows. Summer heat stress depression in fertility is a multifactorial problem that affects physiological and cellular functions in several tissues. It has been shown that in addition to compromise follicular development, hormonal secretion, endometrial and embryonic function, heat stres...
The relationship of plasminogen activator (PA) production to cell stage, cell number and changes in overall diameter and zona pellucida thickness for bovine embryos developing in vitro was determined. Late morulae to blastocysts (n = 80) were collected nonsurgically from naturally mated, estrous-synchronized, superovulated crossbred beef cows. Embryos were cultured, one embryo per 25-microliter...
Background The aim of this study was to establish a cell-free sequential culture system that can support high levels of in vitro embryo development and blastocyst formation from bovine zygotes. To this end, this investigation was carried out to evaluate the effects of glucose, serum and EDTA on bovine zygote in vitro development. MaterialsAndMethods Bovine presumptive zygotes were derived from ...
Abstract Most early human embryos contain aneuploid cells. Aneuploidy often arises during the mitotic divisions of embryo, but its origin remains elusive. Human zygotes that cluster their nucleoli at pronuclear interface are thought to be more likely develop into healthy euploid embryos. I will present data showing how parental genomes with in each pronucleus and bovine zygotes. Parental genome...
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