A novel detection method for the cardiac glycosides digitoxin, digoxin, and their metabolites is described. Both isocratic and gradient water-acetonitrile elutions were employed with an octadecylsilane column. The effluent was monitored by integrated pulsed amperometry in an alkaline medium (pH> 12) by adding sodium hydroxide post-column. Using isocratic elution, peak responses were linear from...

1. Bilateral compression of adrenal glands combined with unilateral nephrectomy and followed by imposition of a high sodium chloride intake caused severe hypertension in all rats, accompanied by enlargement of the heart, kidneys and adrenal glands, atrophy of the thymus and severe nephrosclerosis. 2. Digitoxin treatment delayed the onset, reduced the incidence and ameliorated the magnitude of t...