Background: Digitoxin, in concentrations therapeutic for treatment of cardiac disease, induces apoptosis in different human malignant cell lines in vitro. Our intention with the present study was to investigate if patients on digitoxin for cardiac disease have different cancer incidence compared to the general population. Methods: Computer stored data on digitoxin concentrations in plasma in 92...

Purpose: Digitoxin is a cardiac glycoside used in the treatment of heart failure. Inspired by its known anti-inflammatory effect, this study aims to investigate effect digoxin sepsis model and bring light underlying mechanism acute lung injury (ALI). Method: 28 wistar albino rats were divided into 4 groups. Sepsis performed feces intraperitoneal-injection procedure (FIP). Results: TNF-a, CRP, I...

Prostate cancer is the most occurring form of cancer in men in Sweden and new candidates for treatment towards advanced phases of prostate cancer needs to be investigated. One suggested treatment is vitamin D which will mediate effect via VDR and PDIA3 and cause cell cycle arrest. Another treatment is digitoxin which will cause accumulation of intracellular Ca leading to apoptosis. The aim of t...

Absorption of digitoxin «us studied in 14 fasted anesthetized dogs after administration of 50 (JLC of H-digitoxin mixed with 1 mg of unlabeled digitoxin into either the duodenum or stomach. Radioactivity was measured in portal, hepatic, and central venous plasma, thoracic duct lymph, common duct bile and urine for 5 hours after digitoxin was given. Three dogs were given a single intravenous dos...

Digitoxin and other cardiac glycosides are important, centuries-old drugs for treating congestive heart failure. However, the mechanism of action of these compounds is still being elucidated. Calcium is known to potentiate the toxicity of these drugs, and we have hypothesized that digitoxin might mediate calcium entry into cells. We report here that digitoxin molecules mediate calcium entry int...

Serum concentrations of digitoxin and digoxin were measured in 145 cases with various heart diseases receiving maintenance doses of digitalis. Digitalis toxicity was seen in only 2 cases (1.4%). Day-to-day variation of serum concentration while taking the same daily dose was small in digitoxin therapy (13.8%), but a considerable variation was seen in digoxin therapy (24.4%). Serum concentration...

The effects of Fab fragments of high-affinity specific antibodies have been studied in a canine experimental model of lethal digitoxin toxicity. Selected antiserum from sheep immunized and boosted with a digoxin-serum albumin conjugate contained antibodies that cross-reacted with digitoxin with an average intrinsic association constant of 1.4 x 10(10) M(-1) as determined by equilibrium dialysis...

The nature of digitoxin fixation in isolated hearts was studied with C14 labelled digitoxin. Fixation wvas greatest in the early stages of perfusion. The amount of drug fixed was measured directly. A considerable percentage cf the digitoxin in cardiac muscle was changed to other substances. These substances are more firmly bound to the heart than digitoxin. T HE preparation of pure, randomly la...

New therapies are required for melanoma. Here, we report that multiple cardiac glycosides, including digitoxin and digoxin, are significantly more toxic to human melanoma cells than normal human cells. This reflects on-target inhibition of the ATP1A1 Na(+)/K(+) pump, which is highly expressed by melanoma. MEK inhibitor and/or BRAF inhibitor additively or synergistically combined with digitoxin ...

A growing body of evidence indicates that digitoxin cardiac glycoside is a promising anticancer agent when used at therapeutic concentrations. Digitoxin has a prolonged half-life and a well-established clinical profile. New scientific avenues have shown that manipulating the chemical structure of the saccharide moiety of digitoxin leads to synthetic analogs with increased cytotoxic activity. Ho...