Abstract. Dynamic nuclear polarization (DNP)-enhanced magic angle spinning (MAS) magnetic resonance (NMR) of biological systems is a rapidly growing field. Large signal enhancements make the technique particularly attractive for signal-limited cases, such as studies complex assemblies or at natural isotopic abundance. However, spectral resolution considerably reduced compared to ambient-tempera...

A series of non-aqueous solvents combined with the exogenous biradical bTbK are developed for DNP NMR that yield enhancements comparable to the best available water based systems. 1,1,2,2-tetrachloroethane appears to be one of the most promising organic solvents for DNP solid-state NMR. Here this results in a reduction in experimental times by a factor of 1000. These new solvents are demonstrat...

The concept of shared governance was used to frame the dialogue and decision-making process around the Doctor of Nursing Practice (DNP) at a leading School of Nursing. This article reports the process used to support dialogue around the DNP so that an informed decision could be made. The shared governance process promoted reflective conversation within the entire faculty and, in the end, suppor...

Background: Phenol and nitrophenol are common compounds found in different types of industrial wastewater known as serious threats to human health and natural environment. In this study, Daphnia magna was used to evaluate the effectiveness of "baffle fixes film up flow sludge blanket filtration" (BFUSBF) system in elimination of phenolic compounds from water. Methods: D. magna cult...

The ability of the liver, small intestine, and kidney to synthesize and subsequently eliminate dinitrophenyl-S-glutathione (DNP-SG), a substrate for multidrug resistance-associated protein 2 (Mrp2), was assessed in rats treated with glucagon-like peptide 2 (GLP-2, 12 g/100 g b.wt. s.c. every 12 h for 5 consecutive days). An in vivo perfused jejunum model with simultaneous bile and urine collect...

Previous work has shown that the peptide-like growth factor strepogenin probably occurs in certain proteins such as insulin and trypsinogen at the amino end of the protein (l), and that it is liberated by suitable enzymic digestion. This conclusion suggested a means for attacking the almost insurmountable task involved in separating the one compound strepogenin from the mixture of numerous pept...

This is the third paper in a series (Olah, T. V., Olson, H. M., Glitz, D. G., and Cooperman, B. S. (1988) J. Biol. Chem. 263, 4795-4800; Olson, H. M., Olah, T., Cooperman, B. S., and Glitz, D. G. (1988) J. Biol. Chem. 263, 4801-4806) describing the use of 2,4-dinitrophenyl (DNP) derivatives of Escherichia coli 30 S ribosomal proteins to locate the positions of these proteins within the 30 S sub...

The maturation of B-lymphocyte function during fetal development was studied in vivo and in an in vitro organ culture system. The results indicated that the progenitors for 2,4-dinitrophenol (DNP)-specific B cells are present as early as 14 d of gestation in liver and possibly as early as 15 d in spleen. In addition, it was found that the organ culture system supports the development of B lymph...

In response to a second injection of rabbits with a dinitrophenylated antigen, given 2 months to 2 yr after the first injection, there was rapid synthesis of large amounts of antibody high in relative affinity for the dinitrophenyl (DNP) determinant. The antibodies formed 3 days after restimulation were already high in affinity. Amounts of antigen too small to elicit detectable antibody product...