introduction: the acrosome is a sac shaped organelle contaning lysosomal enzyme that is located on the anterior of sperm head. during the acrosome reaction its content is released in the vicinity of the egg. the acrosome reacted sperm then penetrates through the egg barriers. there are several methods for evaluating the acrosomal status; some of those are complex, expensive and/or non-accurate ...

The aim of this study was to test the hypothesis that the Asp9Asn substitution and the T(293)3G mutation in the promoter of the lipoprotein lipase gene affect plasma lipid levels and thereby the risk of ischemic heart disease (IHD). We genotyped 9033 men and women from a general population sample and 940 patients with IHD. The frequency of both the G allele and the Asn9 allele in the general po...

Clinical tests based on primer-initiated amplification of specific nucleic acid sequences achieve high levels of sensitivity and specificity. Despite these desirable characteristics, these tests have not reached their full potential because their complexity and expense limit their usefulness to centralized laboratories. This paper describes a device that integrates sample preparation and loop-m...

Nguyen MK, Ornekian V, Butch AW, Kurtz I. A new method for determining plasma water content: application in pseudohyponatremia. Am J Physiol Renal Physiol 292: F1652–F1656, 2007. First published February 13, 2007; doi:10.1152/ajprenal.00493.2006.—Pseudohyponatremia is a clinical condition characterized by an increased fraction of protein or lipid in plasma, thereby resulting in an artificially ...

a rapid and sensitive liquid chromatography–tandem mass spectrometry (lc-ms) method for the estimation of enalapril and enalaprilat in human plasma. detection of analytes was achieved by tandem mass spectrometry with electrospray ionization (esi) interface in positive ion mode was operated under the multiple-reaction monitoring mode. sample pretreatment involved in a one-step protein precipitat...

Many of our investigations involving adrenal physiology have been carried out in the rat (14). Since corticosterone is the preponderant adrenal steroid found in rat plasma, l it became essential to develop a rapid, valid method for its determination. The Silber-Porter method (5) is inadequate for studies in the rat because it is neither sensitive nor specific enough. The method of Sweat (B), al...

Isothermal nucleic acid amplification of target RNA or DNA sequences is accomplished by the simultaneous enzymatic activity of AMV reverse transcriptase, T7 RNA polymerase and RNase H. Amplification factors of the nucleic acid sequence based amplification (NASBA) method range from 2 x 10(6) to 5 x 10(7) after 2.5 h incubation at 41 degrees C. During NASBA there is a major accumulation of specif...

1. A novel use of a double-isotope method is described which allows radioenzymatic assays to combine precision and sensitivity. 2. In the catechol O-methyltransferase assay separate portions of each plasma sample are incubated with either S-[3H]- or S-[14C]-adenosyl-L-methionine. Standards of noradrenaline and adrenaline are added to the latter portions and are thus converted into standards of ...