PURPOSE This study was conducted in order to determine the occurrence of esp and biofilm formation among Enterococcus faecalis causing mastitis isolated from different bovine and environmental origins. MATERIALS AND METHODS A total of 41 E. faecalis isolates were obtained from clinical mastitis before antibiotic therapy, subclinical mastitis, dried manure bedding samples, and postpartum milk ...

OBJECTIVES The enterococcal surface protein gene, esp, is a major putative pathogenicity marker in clinical isolates of Enterococcus faecium and Enterococcus faecalis. This study demonstrates in vitro conjugative transfer of the esp gene among E. faecium and E. faecalis. MATERIALS AND METHODS Enterococcal isolates from clinical samples, positive for esp, were mated on filters with enterococca...

Removing the bacteria, including Enterococcus faecalis, from the root canal is one of the important aims in endodontic treatment.We aimed to compare the antibacterial activity of Chlorhexidine with two natural drugs. The antibacterial activities of three different propolis extracts (alcohol concentrations: 0, 15, 40%) and Aloe vera gel on E. faecalis were compared using three methods: disk dif...

The prevalence of acquired resistance in 146 Enterococcus faecium and 166 Enterococcus faecalis strains from farm and pet animals, isolated in 1998 and 1999 in Belgium, against antibiotics used for growth promotion and for therapy was determined. Acquired resistance against flavomycin and monensin, two antibiotics used solely for growth promotion, was not detected. Avoparcin (glycopeptide) resi...

BACKGROUND During the last decade, enterococci have become important nosocomial pathogens, representing the second leading cause of urinary tract infections. This increasing prevalence has been paralleled by the occurrence of multi-drug resistant (MDR) and high-level gentamicin resistant (HLGR) strains. METHODS From September 2005 to 2006, a total of 638 enterococcal isolates were collected f...

introduction: root canal treatment is based on elimination of intraradicular microorganisms. calcium hydroxide ca(oh) 2 is the most widely used material for canal dressing, however, enterococcus faecalis has been reported to be resistant bacteria in persistent apical periodontitis . the purpose of this study was to evaluate the efficacy of ca(oh) 2 on the elimination of intraluminal and intratu...

introduction: enterococcus faecalis ( e. faecalis ) has the ability to invade the dentinal tubules and resist high ph levels. as a result, calcium hydroxide (ch) is not much effective on this bacterium. in theory, nanoparticle calcium hydroxide (nch) has smaller size and high surface area that enables it to penetrate into the deeper layers of dentin and be more effective on e. faecalis. this in...

INTRODUCTION The aim of this in vitro study was to evaluate the antimicrobial activity of four intracanal medicaments on Enterococcus Faecalis (E. Faecalis). METHODS AND MATERIALS Fifty extracted single-rooted human teeth were prepared with standard method. After contaminating the canals with E. Faecalis, the samples were divided into one control and four experimental groups (n=10). The teeth...

We have cloned the DNA gyrase and topoisomerase IV genes of Enterococcus faecalis to examine the actions of quinolones against E. faecalis genetically and enzymatically. We first generated levofloxacin-resistant mutants of E. faecalis by stepwise selection with increasing drug concentrations and analyzed the quinolone resistance-determining regions of gyrA and parC from the resistant mutants. I...

BACKGROUND Enterococci are indigenous flora of the gastro-intestinal tracts of animals and humans. Recently, interest in two major species, E. faecium and E. faecalis, has heightened because of their ability to cause serious infections and their intrinsic resistance to antimicrobials. This study was aimed at determining the prevalence of E. faecium and E. faecalis in human faecal samples and ev...