BACKGROUND Fecal antigen detection using the enzyme linked immunosorbent assay (ELISA) and oocyst detection using auramine phenol (AP) staining methods, are told to be more sensitive compared to other conventional methods, for diagnosis of cryptosporidiosis. OBJECTIVES The aim of the present study was to evaluate the antigen-detection capacity in the stool specimens using ELISA and oocyst det...

An easy and rapid ELISA system, Filtration ELISA, to detect antibodies against bacterial cell surface antigens was developed using a 96-well filtration plate fitted with a 0.22microm membrane (MultiScreen-GV, Millipore). Bacterial whole cells were used as antigens without fixing the cells with formalin etc. The whole cell antigens were washed by vacuum filtration through a filter and resuspende...

fast enzyme linked immunosorbent assay (fast-elisa) was compared with the standard elisa for the diagnosis of human hydatidosis. seventy serum samples including 30 from hydatidosis patients (surgically confirmed), healthy control individuals not infected with any parasitic diseases (n=/20) and from others with different parasitic infections including, toxocariosis (n=5), fasciolosis (n=5), tric...

the aim of this study was to evaluate aflatoxin contamination in feed and milk in dairy farm in kermanshah. alfalfa, corn silage and milk samples from 9 industrial and semi industrial dairy farms were collected during 2 months. blood samples were collected via tail venipuncture from 10 % of dairy cows in each farm for biochemical and hematological parameters. aflatoxin contamination in feeds an...

Introduction: Leptospirosis is a zoonotic disease found in all tropical and temperate areas of the world. Humans contract leptospira through mucosal or percutaneous exposure to environments contaminated by the urine of chronically infected animal sources mainly rodents, dogs, pigs and cattle. Objectives: It is known that IgM ELISA is used widely to diagnose leptospirosis in acute stage of illne...

BACKGROUND AND OBJECTIVES Brucellosis is one of the most common zoonotic diseases in Iran and human brucellosis is endemic in all parts of the country. Because of the difficulty in the diagnosis of brucellosis, particularly in endemic areas, the use of new and feasible diagnostic tests seem to be of great importance for resolving the diagnostic obstacles. We evaluated the usefulness of a new se...