BACKGROUND Since the identity of the embryo is of outmost importance during commercial in vitro embryo production, bovine oocytes and embryos have to be cultured strictly per donor. Due to the rather low yield of oocytes collected after ovum pick-up (OPU) per individual cow, oocyte maturation and embryo culture take place in small groups, which is often associated with inferior embryo developme...

BACKGROUND Characteristics routinely used to evaluate embryo quality after thawing include number of blastomeres survived and presence of mitosis resumption after overnight culture. It is unknown to which extent symmetry and fragmentation affect implantation after warming and whether application of stricter criteria either before vitrification or after warming would improve implantation rate (I...

We developed an air-liquid interphase culture procedure for mammalian oviduct epithelial cells leading to the formation of functional epithelial tissues, which generate oviduct fluid surrogates. These in vitro oviduct epithelia can be co-cultured with living zygotes and enable embryonic development up to the blastocyst stage without addition of embryo culture medium. The described strategy is b...

Patients with repeated implantation failures or poor embryo quality may benefit from embryo culture using the co-culture technique; growth factors secreted by co-culture cells may act as survival factors. Autologous endometrial co-culture has been suggested as a safe alternative to animal cells for co-culture of human embryos. However, the technique is fairly labour intensive and its effectiven...

In recent years, microfluidics has demonstrated novel methods of culturing and studying cells. Within welldefined volumes comparable to the sample of interest, media, nutrients or toxins may be precisely targeted to individual cells via diffusion or advection, enabled by integrated valves and pumps. Tools, which both enable a repeatable and systematic means of single embryo culture, as well as ...

Cell-associated preparations of several isolates of Marek's disease virus produced more pocks on the chorioallantoic membrane of embryonated chicken eggs than plaques in duck embryo fibroblasts, thus indicating that lesion response in eggs was more sensitive than cytopathic response in duck embryo fibroblasts for assaying low-passage Marek's disease virus. Adaptation of the virus to cell cultur...

To further optimize the culturing of preimplantation embryos, we undertook metabolomic analysis of relevant culture media using capillary electrophoresis time-of-flight mass spectrometry (CE-TOFMS). We detected 28 metabolites: 23 embryo-excreted metabolites including 16 amino acids and 5 media-derived metabolites (e.g., octanoate, a medium-chain fatty acid (MCFA)). Due to the lack of informatio...

Embryo culture were used to produce inter specific walnut hybrid plants between Juglans nigra L. and J. regia L. Developed ovaries were collected from J. rejia female flowers which were pollinated with J. nigra pollen grain by high dusting on a mature tree. In order to isolate embryos, exocarps were aseptically removed. Isolated embryos from ovules at different developmental stages were transfe...

PROBLEM Implantation failure is common in women with advanced maternal age, partly because of the increased number of aneuploid embryos. These women constitute the majority of patients for IVF treatment. As multiple pregnancies is a major hazard of preterm delivery, the aim is to select a competent embryo for single transfer. This study reviews currently used methods for selecting the competent...

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