This is a compilation of reference materials suggested for scientists considering a laboratory reference collection in clinical chemistry, or for librarians seeking to develop or add to an up-t-o-date library collection. Nine areas are represented: general clinical chemistry; analytical methods, procedures, and techniques; calculations and statistics; the clinical laboratory; drugs and metaboli...

Molecular Enzymology Group Colloquium Organized by D. Fell (School of Biological and Molecular Sciences, Oxford Brooke University) and D. Kell (Department of Biological Sciences, University College, Wales, Aberystwyth), Edited by D. Fell and Sponsored by Xenova Ltd, Zeneca Bioproducts, Glaxo Research and Development Ltd and SmithKline Beecham Pharmaceuticals. 653rd Meeting held at the Universit...

MOTIVATION Recent advances in mass spectrometry and related metabolomics technologies have enabled the rapid and comprehensive analysis of numerous metabolites. However, biosynthetic and biodegradation pathways are only known for a small portion of metabolites, with most metabolic pathways remaining uncharacterized. RESULTS In this study, we developed a novel method for supervised de novo met...

Accurate, economical and high-throughput gene and genome synthesis is essential to the development of synthetic biology and biotechnology. New large-scale gene synthesis methods harnessing the power of DNA microchips have recently been demonstrated. Yet, the technology is still compromised by a high occurrence of errors in the synthesized products. These errors still require substantial effort ...

Aim: The study compares and evaluates enzymatic gel Brix 3000â Polymer bur (SS White) for caries excavation in primary molars terms of two parameters such as pain reaction the time taken excavation. Method: 60 children aged 7-9 years who require removal were recruited randomly allocated into groups: Group-A: Enzymatic (Brix 3000)â, Group-B: Smart burs White polymer burs). objectives evaluated w...

An enzymatic system which reduces nicotinamide N-oxide to nicotinamide was isolated from hog liver and purified about 280-fold. The reduction is dependent on the presence of reduced diphosphopyridine nucleotide or a low molecular weight constituent found in boiled liver supernatant. The two cofactors appear to be involved in the expression of two distinct enzymatic activities. Evidence has been...

Introd u ction Clinical chemistry, for decades restricted to a limited number of chemical tests, has, in recent years, through an increasing awareness of the value of the laboratory investigation of disease, and the introduc­ tion of automation and sophisticated instru­ mentation, expanded its field of operations to include a bewildering variety of tests. Advances in biochemical knowledge have ...

enzymatic acidolysis of canola oil through caprylic and capric acids was investigated for making structured lipids. lipozyme tl im, an sn-1,3 specific thermomyce slanuginosa lipase, was utilized as the biocatalyst in the batch reactor. reaction conditions were designed according to taguchi’s method considering fatty acid to oil ratio, at three levels (1:1, 2:1, 3:1) , enzyme load (4, 8 and, 12%...

In this study, the hydrolysis of pretreated sorghum stem and rice husk was investigated at various initial enzyme concentrations and substrate loadings. The slowdown in enzymatic hydrolysis of lignocellulosic materials with conversion has often been attributed to decreasing the activity of enzyme. A kinetic model was developed and expressed mathematically based on enzyme deactivation for enzyma...

Enzymatic inhibition is one of the key regulatory mechanisms in cellular metabolism, especially enzymatic competitive by product. This process helps cell regulate activities. In this paper, we derive a mathematical model describing The consists coupled system nonlinear ordinary differential equations for species interest. Using nondimensionalization analysis, formula product formation rate mech...