The snake venom´s thrombin-like enzymes comprise a number of serine proteases, which are functionally and structurally related to thrombin. Purification and partial characterization of a thrombin-like enzyme from the venom of the Iranian snake, Agkistrodon halys, was the aim of this study. Purification was carried out by a combination of variety of chromatographic methods that included: gel...

A novel neutrophilic metalloprotease was isolated from Pseudomonas sp. DR89 isolate which was identified ina mineral spring in Iran. The enzyme was purified from the isolate to 21-folds in a three-step procedure involving ammonium sulfate precipitation, Q-Sepharose ionic exchange and Sephadex G-100 gel filtrationchromatography. Resuts showed that the enzyme was active at high temper...

AaCel9A [β-1,4-endoglucanase, (E.C:3.2.1.4)], was immobilized onto glutaraldehyde activated chitosan macrosphere by covalent attachment. The properties of the immobilized AaCel9A were investigated by determining the optimum pH and optimum temperature for activity, thermal stability, and kinetic parameters. The immobilization process shifted the enzyme’s optimum temperature from 65 °C for the fr...

Enzyme mimetic is an increasing pull to overcome the limitations on natural enzymes. mimicking done with help of nanozymes. Nanozymes contain nonmaterial which have enzyme-like activity; this similar activity attracts researchers.

introduction corn has been recently used for producing ethanol fuel in the major corn-producing countries such as the us and brazil. recent diversion of corn for biofuel production along with the increased world's demand for this feedstuff has resulted in unprecedented rise in feed cost for poultry worldwide. alternative grains such as wheat and barley can be successfully replaced for corn in p...

the mechanism by which bi-and trivalent cations affect human liver phosphatidatephosphohydrolase (pap) activity was investigated. bivalent cations up to 1 mm increased pap activity whereas at higher concentrations the activity of the enzyme decreased. the stimulatory concentration for trivalent cations such as al3+ and cr3+, however, was much lower being 2 m m and 1 m m, respectively. all catio...

leptinotarsa decemlineata (say) and lasioderma serricorne f. are destructive pre-harvest and post-harvest pests of many plants in the family solanaceae, and stored foodstuffs and non-food items, respectively. in this study, some biochemical characteristics of cellulase in the larval digestive tract of these pests were studied. endo-β-1, 4-glucanase activity was measured against the substrate ca...