alpha toxin derived from clostridium perfringens was used for diagnosis of streptococcus agalactiae (st.agalactiae) by rapid and spot camp tests. the selected cl.perfringens strain was cultured in optimized conditions; the supernatant of culture was concentrated by ultra filter and purified by deae cellulose chromatography. the efficacy of both purified and crude α-toxins were examined by rapid...

the effect of epinephrine on phosphatidate phosphohydrolase (pap) activity of isolated human hepatocytes was studied. epineprine inhibited the enzyme activity progressively at concentrations above 0.1 µm, reaching a maximum inhibition of 64.5% at 100 µm concentration. inclusion of alprenolol, a β-receptor blocker, in the incubation mixture abolished the inhibitory effect of epinephrine on pap, ...

lycopene is a natural and dominant pigment in tomato with coloring and antioxidant properties, which nowadays the demands for its extraction enhancement from tomato and tomato products are increasing. in this study, the effect of pectinase enzyme pre-treatment at 2- 10 ml/kg and also washing with 94% ethanol at 60°c for 5 seconds was studied. in these conditions, treating with 10 ml/kg enzyme c...

plants are naturally exposed to frequent changes in environmental conditions such as salt stress affecting their growth and development, and consequently a considerable reduction in total productivity. monodehydroascorbate reductase (mdhar), a key enzyme in ascorbate-glutathione cycle, serves as an important antioxidative enzyme in scavenging of reactive oxygen spices (ros). in this study, mdha...

Nine Aspergillus and three of Trichoderma strains were grown on wheat bran (WB) medium under solid state fermentation (SSF) for amylase production. Aspergillus oryzae LS1 produced the highest level of the enzyme. The thermal stability profile of its crude enzyme revealed the half-life time of more than 2 h at 50 and 60oC. The enzyme production was affected by strain type, incubation periods, le...