background: this study was undertaken to establish the characterization of cultured oral mucosal epithelium and introducing them as an alternative source for reconstruction of ocular surface disease. methods: human oral epithelial cells were cultured on simple media (dmem/hf12) as control and co-cultured on mitomycin c-treated 3t3 feeder layer, on the amniotic membrane (am) without nitrocellulo...

Epithelial plasma membranes from crustacean gut, kidney and gills have been shown recently to display an electrogenic 2Na+/1H+ antiporter that differs considerably in its physiological properties from the vertebrate electroneutral 1Na+/1H+ exchange paradigm. In this study, we describe the histological and cytological localization of an antigen associated with invertebrate electrogenic 2Na+/1H+ ...

24 Invasion of intestinal epithelial cells by Salmonella enterica serovar Typhimurium 25 is an energetically demanding process, involving the transfer of effector proteins from 26 invading bacteria into host cells via a specialized organelle known as the SPI-1 type 327 secretion system (T3SS). By a mechanism that remains poorly understood, entry of S. 28 Typhimurium into epithelial cells is inh...

The developmental pattern of appearance of a structural corneal antigenic determinant was studied by immunofluorescent methods. It was first detected in the primary corneal stroma at stage 23, and thereafter spread throughout the primary and secondary stroma. This antigen was absent from Bowman's membrane at its initial stage of development, suggesting that this membrane is a new structure in c...

Abstract It has become increasingly evident that even though B cells respond to both soluble and membrane-presented antigens in vitro, the primary mode of antigen recognition vivo appears be on surfaces antigen-presenting cells. To determine how sense an is membrane associated, we investigated role Piezo1 mechanosensing cation channel human B-cell activation. We discovered flux calcium when sim...

A layer of amorphous, electron-dense material is situated at the cytoplasmic surface of the postsynaptic membrane of vertebrate neuromuscular synapses. The function of this structure is not clear, but its location suggests that it may have an important role in the formation and/or maintenance of the synapse. This paper demonstrates that a monoclonal antibody raised against antigens from Torpedo...

A case of a rare benign solitary pulmonary neoplasm of alveolar adenoma type in a 42-year-old man was presented. The tumor consisted of a glandular component which reacted with antibodies against cytokeratins CAM5,2, AE1-3 as well as against epithelial membrane antigen (EMA) in superficial parts of cytoplasmatic membrane, while actin and desmin were positive in the stromal component. Ultrastruc...

The human amniotic membrane known as the innermost single epithelial-covered layer provides many applications such as applicable anti-inflammatory and anti-cancer effects. These immunomodulatory effects belongs to the epithelial cells, a type of epiblast-derived fetal stem cells which currently used for regenerative medicine and transplantation. These cells are collected by author-prepared faci...

Neuroblastoma (NB) cells possess morphological and biological properties in vivo and in vitro, the cell type having the characteristics of primitive sympathetic neuroblasts (Beckwith et al., 1963; Biedler et al., 1981; Kissel et al., 1981; Ross et al., 1981). NB cell lines established in vitro have been shown to consist of two morphologically distinct types of cells: neuroblast-like and epithel...