The objective of the present study was to investigate the mobilization of individual fatty acids from human white fat cells. Mammary adipose tissue from eight healthy non-obese women in their normal dietary state was collected, and isolated adipocytes were incubated with lipolytic agents. The mobilization of 34 individual fatty acids was measured by comparing the composition of non-esterified f...

We developed a method for determining individual free fatty acids in serum by using a modified one-step Dole extraction, derivatization, and a new high-performance liquid chromatographic (HPLC) separation. Sample handling is minimized to a single transfer of the fatty acids (upper layer of the Dole extract), which are readily derivatized at 85 degrees C with p-bromophenacyl bromide without sign...

Differential effects of fatty acids on carcinogenesis suggest that fatty acid composition is important in tumor development. Arachidonic acid and its metabolites elicit inflammation and promote tumor formation in mouse skin. Inhibitors of the arachidonic cascade inhibit tumor incidence. A population-based case control study in Southeastern Arizona tested the hypothesis that lower levels of arac...

The present study evaluated the effects of diets enriched in saturated and polyunsaturated fatty acids (n-3and n-6) on reproductive indices, metabolic hormones and metabolites prior to ram introduction in oestrussynchronized ewes. Zel ewes (n=188) were assigned to 4 groups. Ewes in the control group (CON) did notreceive fat. Ewes in the 3 other groups received 3% oil/DM/day of palmolein oil (PL...

Inconsistencies in feeding level of dairy cattle to their feed needs leads various metabolic diseases, including ketosis. The purpose our work is study changes the metabolism both sexually mature representatives and young animals obtained from them. article states following causal factors ketosis occurrence cows: excessive amount fat substances, imbalance chemical elements diet. pathology carbo...

Algal preparations from Acetabularia crenulata were analyzed for their fatty acid composition to establish the suitability of this alga as a model to study fatty acid oxidation and oxylipin biosynthesis. The work was based on two goals. The first goal of this study was to determine the contribution of fatty acids from contaminating bacteria and how this influenced the total fatty acid compositi...

To facilitate the study of adrenoreceptor response in small needle biopsy samples of human subcutaneous adipose tissue, we developed a dual radioisotopic technique for measuring lipolysis rate. Aliquots (20-75 mg) of adipose tissue fragments were incubated in a buffered albumin medium containing [3H]palmitate and [14C]glucose, each of high specific activity. In neutral glycerides synthesized in...

To investigate the relative turnover of esterified polyunsaturated fatty acids in diacylglycerophospholipids and plasmalogens in isolated cardiac myocytes, we characterized the phospholipid composition and distribution of radiolabel in different phospholipid classes and in individual molecular species of diradyl choline (CGP) and ethanolamine (EGP) glycerophospholipids after incubation of isola...

Studies both in vitro and in vivo have established that the hydrolysis of triglycerides in the intestinal tract can yield diglycerides, monoglycerides, free fatty acids, and free glycerol. Thus far it has not been possible to establish which of these materials are absorbed as such into the intestinal wall. That a solution to t.his problem has not been forthcoming was undoubtedly attributable to...

Exogenous [1-(14)C]oleic acid and [1-(14)C]linoleic acid were taken up and esterified to complex lipids by greening cucumber (Cucumis sativus L.) cotyledons. Both (14)C-labeled fatty acids were initially esterified to phosphatidylcholine prior to eventual accumulation in triacylglycerols and galactolipids. Kinetic data suggest that esterification occurs prior to desaturation and that phosphatid...