BACKGROUND The amyloid beta (Abeta) peptide is a key molecule in the pathogenesis of Alzheimer's disease. Reliable methods to detect and quantify soluble forms of this peptide in human biological fluids and in model systems, such as cell cultures and transgenic animals, are of great importance for further understanding the disease mechanisms. In this study, the application of new and highly spe...

Objective: To determine the effect of convallatoxin on K562 cell proliferation and apoptosis. Methods: CCK-8 assay was used to detect proliferation; PI staining, JC-1 Annexin V-FITC/PI double staining were analyze cycle, mitochondrial membrane potential, apoptosis; Western blotting cleaved caspase-9, caspase-3, Bcl-2, Bax, E2F1 expression Akt phosphorylation. Subsequently, AutoDock software int...

Glioblastoma is most common and most aggressive cancer of brain. for treatment surgery is first selection and radiation therapy then chemotherapy. The median survival of Patients with GBM is less than a year after diagnosis. Glioblastoma is basically resistant to common cancer treatments. today to improve the response of patients to treatment, a number of strategies such as the use of radiation...

in this paper we will prove that the simple group g2(q) where 2 < q = 1(mod3)is recognizable by the set of its order components, also other word we prove that if g is anite group with oc(g) = oc(g2(q)), then g is isomorphic to g2(q).

Purpose We previously found that the histone methyltransferase suppressor of variegation, enhancer of zeste, trithorax and myeloid-nervy-deformed epidermal autoregulatory factor-1 domain-containing protein 3 (SMYD3) is a potential independent predictive factor or prognostic factor for overall survival in gastric cancer patients, but its roles seem to differ from those in other cancers. Therefor...

OBJECTIVES MiRNAs are intrinsic RNAs that interfere with protein translation. Few studies on the synergistic effects of miRNAs have been reported. Both miR-424 and miR-381 have been individually reported to be involved in carcinogenesis. They share a common putative target, WEE1, which is described as an inhibitor of G2/M progression. Here, we studied the synergistic effects of miR-424 and miR-...

not only cancer cells but also normal cells. Because these drugs are not specifically selective for cancer cells, patients suffer from adverse side effects due to the loss of healthy cells. Therefore, it is important to reduce such side effects caused by anti-cancer drugs. DNA damage induces signal transduction pathways called checkpoints, which delay cell cycle progression and permit repair of...

Point mutations of the active-site residues Trp168, Tyr171, Trp275, Trp397, Trp570 and Asp392 were introduced to Vibrio carchariae chitinase A. The modeled 3D structure of the enzyme illustrated that these residues fully occupied the substrate binding cleft and it was found that their mutation greatly reduced the hydrolyzing activity against pNP-[GlcNAc](2) and colloidal chitin. Mutant W397F wa...

The main aim of this study was to obtain the Crimean-Congo hemorrhagic fever virus (CCHFV) glycoprotein, through either stable transgenic plants or using a transient expression system, and determine the yield, quality and finally the immunogenicity of the plant-made CCHFV glycoprotein in a mouse model. We designed and synthesized a codon-optimized G1/G2 gene from the G1 and G2 parts of the CCHF...