The study aimed at direct detection of Mycobacterium avium subsp. Paratuberculosis (MAP) in milk by evaluating a multiplex real-time PCR assay targeting IS900 and ISMAV2 sequences including the amplification of PUC19-plasmid as internal control. The sensitivity of the assays was evaluated by testing MAP isolates in broad linear range of DNA (50 ng – 5 fg/μl). For the validation of the specifici...

The aim of this study was to evaluate the reliability of flow cytometry analysis and the use of this technique to differentiate species and varieties of sugarcane (Saccharum spp) according to their relative DNA content. We analyzed 16 varieties and three species belonging to this genus. To determine a reliable protocol, we evaluated three extraction buffers (LB01, Marie, and Tris·MgCl2), the pr...

methylated and un-methylated control dna is an important part of dna methylation studies. although human and mouse dna methylation control sets are commercially available, in case of methylation studies on other species such as animals, plants, and bacteria, control sets need to be prepared. in this paper a simple method of generating methylated and un-methylated control dna is described. whole...

Medicinal plants have high importance in medicine and pharmacogonosy because of their secondary metabolites. Essential oils, antioxidants and flavonoids are the major compounds of many medicinal plants. These compounds especially antioxidants can increase oxidation of DNA in response to wounds. Nowadays, progresses in biotechnology such as molecular markers were used for phylogenetic relationsh...

Genome editing technology can be used to extensively modify genetic information in DNA, and such applications are expected widely applied the fields of medicine agriculture. In chickens, genome primordial germ cells has made it possible create genome-edited which may lead improvements breeds that have been difficult achieve using conventional breeding methods. The development products derived f...

Genomic DNA represents the total genome of an organism and is valuable for different molecular studies. The isolation of high quality DNA is a prerequisite for these molecular techniques. The seeds of apple (Malus domestica) contain high amount of polysaccharides, polyphenols and other secondary metabolites that can hamper DNA isolation, amplification, restriction digestion and subsequent molec...

dna sequence determination is a tremendous human achievement. dna sequencing includes several methods and technologies in use for determining the order of the nucleotide bases (adenine, guanine, cytosine, and thymine) in a molecule of dna. knowledge of dna sequences has become indispensable for basic biological research, other research branches utilizing dna sequencing, and in numerous applied ...

chlamydia trachomatis is the most common agent of urogenital infections in both men and women. diagnosis of chlamydial infections is based on isolation of bacteria in tissue culture media that requires at least 48 to 72h. polymerase chain reaction (pcr) is a sensitive and specific method for detection of small quantity of bacterial dna in clinical samples. the first goal of this study was to pe...

Abstract Until there is an effective implementation of COVID-19 vaccination program, a robust testing strategy, along with prevention measures, will continue to be the most viable way control disease spread. Such strategy should rely on disparate diagnostic tests prevent slowdown in due lack materials and reagents imposed by supply chain problems, which happened at beginning pandemic. In this s...

The Varicella-Zoster Virus (VZV) genome contains both cis-acting and trans-acting elements, which are important in viral DNA replication. The cis-acting elements consist of two copies of oriS, and the trans-acting elements are those genes whose products are required for virus DNA replication. It has been shown that each of the seven genes required for ori-dependent DNA synthesis of Herpes Simpl...