Most investigations on the kinetics of hemolysis have been based on the idea that the lysins enter into irreversible combination with components of the red cell membrane, and imply that the lytic reaction is accompanied by a diminution in the quantity of free lysin present in the system. Attempts at expressing the velocity of hemolytic reactions accordingly contain, in one form or another, the ...

A DNA probe specific for Listeria monocytogenes was isolated from a beta-hemolytic recombinant clone of an L. monocytogenes gene bank. It was labeled with horseradish peroxidase and used in a direct colony hybridization method on hydrophobic grid-membrane filters for the detection of the organism. Following color development of the chromogen, a commercial counter (HGMF Interpreter) was able to ...

Introduction.?Koch in 1883 isolated the cholera vibrio from cholera patients in Calcutta. Tinkler and Prior (1884) and Deneke (1885) described vibrios resembling Koch's vibrio that had been isolated from non-choleraic material. In the twelve years following the discovery of the cholera vibrio, Metchnikof, Sanarelli, Gunter, Foker oftid other workers isolated vibrios indistinguishable morphologi...

In this study, we report the first Korean case of an anti-Gerbich (Ge) alloantibody to a high-incidence antigen that belongs to the Ge blood group system. The alloantibody was detected in a middle-aged Korean woman who did not have a history of transfusion. Her blood type was B+, and findings from the antibody screening test revealed 1+ reactivity in all panels except the autocontrol. The cross...

Sickle cell anemia (SCA) is a genetic disorder characterized by homozygous hemoglobin S (Hb S), chronic hemolytic anemia and painful episodes.1 Patients with SCA usually require red blood cell (RBC) transfusions to manage complications and to reduce morbidity during surgical procedures.1 One possible complication of multiple transfusions is alloimmunization, which occurs due to the recognition ...

By means of the immunofluorescent staining technique, antisera to a wide variety of serological types of Group A and A variant streptococci were found to contain an antibody which reacted with mammalian striated muscle, both skeletal and cardiac, as well as with smooth muscle in the endocardium and in the media of arterioles. Similar heart-reactive antibodies were not present in antisera to mos...

The effects of pristane on some immunity parameters in BALB/c mice were studied. The intraperitoneal administration of a single dose of pristane induced a strong inflammatory reaction that lasted longer than 10 days. When mice were immunized with sheep erythrocytes 10 days after pristane administration, the response of hemolytic IgM-forming cells was increased and that of hemolytic IgG-forming ...

G LUCOSE-PHOSPHATE ISOMERASE (GPI) controls the equilibrium between glucose-6-phosphate (G-6-P) and fructose-6-phosphate (F-6-P). Though G?I does not seem to have a rate-limiting function in red cell glycolysis,’ deficiencies of this enzyme cause hereditary nonspherocytic hemolytic anemia.2 Twelve cases from nine different families have been reported suffering from hemolytic anemia caused by G?...

background: sodium bicarbonate serum therapy is used for compensation bicarbonate lost and increasing blood ph in metabolic acidosis caused by severe anemia in patient with glucose-6-phosphate dehydrogenase (g6pd) deficiency. the aim of present study was comparison the effect of serum therapy using two different serums (serum with bicarbonate and without bicarbonate) on some renal and hematolog...

introduction autoimmune haemolytic anemia (aiha) is a complex process characterized by an immune reaction against red blood cell self-antigens. the analysis of specimens, drawn from patients with cold auto-immune hemolytic anemia is a difficult problem for automated hematology analyzer. this paper was written to alert technologists and pathologists to the presence of cold agglutinins and its ef...