Knowledge of the response of cytochrome P450 1B1 (CYP1B1) to exposure to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) in both humans and rodents is limited. To improve the analysis of CYP1 proteins, specific CYP1B1 and CYP1A1 polypeptides were expressed as hexahistidine-tagged fusion proteins in Escherichia coli, purified to homogeneity and used to produce polyclonal antibodies in rabbits. Immuno...

Tandem affinity strategies reach exceptional protein purification grades and have considerably improved the outcome of mass spectrometry-based proteomic experiments. However, current tandem affinity tags are incompatible with two-step purification under fully denaturing conditions. Such stringent purification conditions are desirable for mass spectrometric analyses of protein modifications as t...

milton friedman’s seminal article, “methodology of positive economics”, is one of the most important and contentious works ever been discussed within the realm of economic methodology. in this article, on the basis of an instrumentalist approach to the nature of theorizing, friedman declares the tenets of positive economics, and in this vein, he professes his disputable doctrine about “unrealis...

the present article studies the reality of “zohdyyat” by abul atahie. has abul atahie been a real pious? or has he chosen piety as a technic for poetry? is his piety islamic? or is it spiritual? or is he an infidel? the present article tries to study the doubts and accusations in this regard so that we will know the reality of his “zohdyyat”. for the sake of this reason we first study the eleme...

Copper coordination to soluble oligomers of the English (AβH(6)R) mutant of the amyloid-β peptide is probed. Cu(II) coordination yields a square planar (N/O)4 coordination environment, while reduction yields an O2 inert linear bis-His Cu(I) centre.

khatib tabrizi is a famous scholar from fifth hijry century. he has been named with positive and negative characteristics. this article studies and judges the things he has been accused of or appraised for. also his scientific status and historian views about him and his role in literature are discussed in the present article.