The inhibition of the spreading effect of hyaluronidase by the previous administration of sodium salicylate was reported by Guerra and Robles Gil (1). Subsequent investigations indicated that this phenomenon could not be explained on the basis of the direct inhibition of hyaluronidase by salicylates, since such inhibition could only be demonstrated in vitro at high salicylate concentrations (2-...

Hyaluronidases degrade hyaluronic acid, which promotes metastasis. HYAL1 type hyaluronidase is an independent prognostic indicator of prostate cancer progression and a biomarker for bladder cancer. However, it is controversial whether hyaluronidase (e.g., HYAL1) functions as a tumor promoter or as a suppressor. We stably transfected prostate cancer cells, DU145 and PC-3 ML, with HYAL1-sense (HY...

An operationally simple colorimetric way for measuring hyaluronidase activity was developed using cysteamine-bound gold nanoparticles. The addition of gold nanoparticles into hyaluronidase-containing solutions resulted in color changes, which could easily be observed with the naked eye or a UV/Vis spectrophotometer.

Mycoplasma alligatoris causes lethal invasive disease of alligators and caimans. A homolog of the nagH gene, encoding a hyaluronidase secreted by Clostridium perfringens, and a C. perfringens hyaluronidase nagI or nagK pseudogene were discovered in the M. alligatoris genome. The nagH gene was detected by PCR in the closest relative of M. alligatoris, Mycoplasma crocodyli, but not in 40 other sp...

hyaluronidase has a panoramic use in biotechnology processes and therapy due to its therapeutic, pathophysiological, physiological and biological importance. since much of the preparations of hyaluronidases are from animal source (bovine and ovine testicular sources) with limited sources of microbial origin, that prompted the authors to screen and isolate a new promising bacterial strain with h...

A variety of methods have been devised for the assay of the enzyme hyaluronidase. These may be divided into four types: (a) measurement of the “spreading effect” in the skin of rabbits and guinea pigs (1, 2), (b) reduction of viscosity (3,4), (c) prevention of mucin clot formation (5), and (d) chemical measurement of total reducing substances, N-acetylglucosamine, or glucuronic acid liberated o...

Prior to ICSI the cumulus-oophorous-complex of the oocyte is commonly remove with impure animal-derived hyaluronidase of low strength. The lower strength of the enzyme, the high concentration of impurities and the necessity of extensive time-dependent mechanical denudation damages of the oocytes, increases the risk of lysing the oocytes and the transfer of animal-derived pathogens. All this can...