The sodium dodecyl sulfate (SDS)-solubilized freeze fracture replica labeling (SDS-FRL) technique has been successfully applied to a vascular plant, Vigna angularis [Kimura et a!. 1999, Plant Cell II: 2075 2085] and a bacterium, Acetobaeter xylinum [Kimura et al. 2001, J. Bacteriol. 183: 5668 -5674] for understanding cellulose biosynthesis. However, we do not know yet how many gold particles ca...

Quantifying concentrations of target molecules near cellular structures, within cells or tissues, requires identifying the gold particles in immunogold labelled images. In this paper, we address the problem of automatically detect them accurately and reliably across multiple scales and in noisy conditions. For this purpose, we introduce a new contrast filter, based on an adaptive version of the...

Mature maize leaf tissue (Zea mays L.) was immunolabeled using a pre-embedding protocol with specific antibodies for nitrate reductase and protein A-colloidal gold. Immunogold label was found exclusively in the cytoplasm of mesophyll cells; no reaction was detected in bundle sheath cells. Chloroplasts, which were sliced open during cryosectioning, had no labeling. Thus, it appears nitrate reduc...

Unequivocal evidence of the viral nature of virus-like particles observed at the specific site of retention of tobacco rattle virus (TRV) in Paratrichodorus and Trichodorus nematodes has not previously been available. A new staining technique using safranin-O, which does not affect viral antigenicity, was used with an antiserum raised against the coat protein of TRV and prepared for use with im...

Labeling nuclear proteins with electron dense probes in living cells has been a major challenge due to their inability to penetrate into nuclei. We developed a lipid-based approach for delivering antibodies coupled to 0.8 nm ultrasmall gold particles into the nucleus to label RNA polymerase II. Focussed Ion Beam slicing coupled to Scanning Electron Microscopy (FIB/SEM) enabled visualization of ...

Abstract Huntington’s disease (HD) is a late onset, inherited neurodegenerative disorder for which early pathogenic events remain poorly understood. Here we show that mutant exon 1 HTT proteins are recruited to subset of cytoplasmic aggregates in the cell bodies neurons brain sections from presymptomatic HD, but not wild-type, mice. This occurred stage and polyglutamine-length dependent manner....

Electron microscopic immunogold labeling experiments were performed with ultrathin sections of plasmolyzed cells of Alcaligenes eutrophus and "whole-mount" samples of spheroplasts and protoplasts. They demonstrated that antigenic determinants of the membrane-bound hydrogenase are exposed, at the outside of the cytoplasmic membrane, to the periplasm.