Freeze Substitution and Low Temperature Processing of Cryo Protected Tissues into Lowicryl HM23 Resin for Correlated Light Immunogold Electron Microscopy in Drug Discovery Safety Assessment Research - Volume 28 Issue S1

G lycosylation, the synthesis of branched sugar chains on proteins, is a posttranslational modifi ca-tion commonly found on membrane and secretory proteins. Glycosylation is important for proper protein folding, and the composition of the sugar chains can also be used to track a glycoprotein's maturation. When Jürgen Roth started working on glycoproteins, researchers had only recently begun pro...

Introduction: The implantation is a complex procedure that involves many molecules. One of these molecules is integrin specially &alphav&beta3 which serves as receptor for components of extra cellular matrix to act as bridging molecules between the blastocyst and the endometrial surface during the implantation process. By blocking &alphav&beta3 interactions, the implantation can be impaired. ...

introduction: the implantation is a complex procedure that involves many molecules. one of these molecules is integrin specially &alphav;&beta3; which serves as receptor for components of extra cellular matrix to act as bridging molecules between the blastocyst and the endometrial surface during the implantation process. by blocking &alphav;&beta3; interactions, the implantation can be impaired...

BACKGROUND Immunogold cytochemistry is the method of choice for precise localization of antigens on a subcellular scale. The process of immunogold quantification in electron micrographs is laborious, especially for proteins with a dense distribution pattern. NEW METHODS Here I present a MATLAB based toolbox that is optimized for a typical immunogold analysis workflow. It combines automatic de...