Abstract Grey oyster mushroom is the most often farmed and marketed type of for household consumption in Malaysia. The cultivation takes a long time due to its complicated mycelium growth. Several treatments were introduced such as acoustic sound treatment increase growth rate quality crop, but there no in-depth study regarding genetic expression genes affected. This aims evaluate Differential ...

a. We recommend that the standard methodology for genetic diagnosis of autosomal dominant polycystic kidney disease is polymerase chain reaction (PCR) amplification (including long-range PCR for the first 33 exons of PKD1) followed by Sanger sequencing (1A) or next-generation sequencing where available (1D). b. We suggest that individuals with a clinical diagnosis of autosomal dominant polycyst...

Introduction Infection is one of the most important causes of joint implant failure. The polymerase chain reaction (PCR) is a sensitive way to test for bacteria, but most PCR procedures take too long to provide results during a revision arthroplasty operation. For example, we have described the sensitivity and specificity of real-time PCR combined with ultrasonication of retrieved implants to d...

We have adapted the originally described electronic PCR (e-PCR) algorithm to perform string searches more accurately and much more rapidly than previously possible. Our implementation [multithreaded e-PCR (me-PCR)] runs sufficiently fast to allow even desktop machines to query quickly large genomes with very large genomic element sets. In addition, me-PCR is multithreaded, interprets all IUPAC ...

Neoadjuvant therapy has become the standard of care for locally advanced mid-low rectal cancer. Pathological complete response (pCR) can be achieved in 12%-38% of patients. Patients with pCR have the most favorable long-term outcomes. Intensifying neoadjuvant therapy and extending the interval between termination of neoadjuvant treatment and surgery may increase the pCR rate. Growing evidence h...

We present a method that allows simultaneous fusion and cloning of multiple PCR products in a rapid and efficient manner. The procedure is based on the use of PCR primers that contain a single deoxyuridine residue near their 5' end. Treatment of the PCR products with a commercial deoxyuridine-excision reagent generates long 3' overhangs designed to specifically complement each other. The combin...

BACKGROUND Neoadjuvant therapy is administered to breast cancer patients as an induction process before surgery or radiotherapy to reduce tumor size. Human epidermal growth factor receptor-2 (HER-2) negative breast cancer lacks effective standard target therapy. Bevacizumab has a controversial role in the treatment of breast cancer and we conduct a meta-analysis to evaluate the value of adding ...

There are a number of applications of molecular markers in agriculture such as assessing genetic diversity, generating DNA fingerprints and developing markers linked to a trait of interest, etc. Arbitrarily Primed-Polymerase Chain Reaction (AP-PCR) in which template DNA is amplified using single arbitrary primers of 10 base in length is a widely used technique. The objectives of this investigat...

Consistent temperature control in an on-chip flow-through polymerase chain reaction (PCR) employing two or more heaters is one of the main obstacles for device miniaturization and integration when realizing micro total analysis systems (μTAS), and also leads to operational complexity. In this study, we propose a qiandu (right triangular prism)-shaped polydimethylsiloxane (PDMS) microdevice with...

abstract         background and objective: paratuberculosis has been repeatedly reported from iranian ruminant herds. the extrem fastidious nature of mycobacterium avium subspecies paratuberculsos hinders genomic diversity studies of the pathogen. short sequence repeat analysis is one of the genome-based approches recently developed to overcome this difficulty. in this study we describe the app...