Quantification of the tissue and cellular structure of plant material is essential for the study of a variety of plant sciences applications. Currently, many methods for sectioning plant material are either low throughput or involve free-hand sectioning which requires a significant amount of practice. Here, we present an updated method to provide rapid and high-quality cross sections, primarily...

Imaging intracellular calcium concentration via reporters that change their fluorescence properties upon binding of calcium, referred to as calcium imaging, has revolutionized our way to probe neuronal activity non-invasively. To reach neurons densely located deep in the tissue, optical sectioning at high rate of acquisition is necessary but difficult to achieve in a cost effective manner. Here...

Improvements in the understanding of the physiology of the central airways require an appropriate representation of the non-uniform ventilation at its terminal branches. This paper proposes a new technique for estimating the non-uniform ventilation at the terminal branches by modelling the volume change of their distal peripheral airways, based on vascular segmentation. The vascular tree is use...

OBJECTIVE To determine the level of sentinel lymph node sectioning necessary to accurately detect nodal micrometastasis. STUDY DESIGN Cross-sectional. SETTING Tertiary care university medical center. SUBJECTS AND METHODS Fine sections of oral squamous cell carcinoma sentinel lymph nodes previously sectioned at 2-mm intervals in a prospective clinical trial were reexamined. The results yie...

In timetabling of large groups we may need to both split events into smaller events, and also to assign students to these events. We investigate the interaction between these from a space planning perspective, and in particular, the effects on the effective conflict matrix.

Abstract In the current study, electrolytic deposition using two different electrodes, copper (Cu) and nickel (Ni) was investigated with aim of protecting worn surface during mechanical sectioning polishing, for a posterior examination sub-surface microstructure. The efficacies coatings were visually assessed based on its adhesivity ability to protect wear tracks an as-cast 26% Cr high chromium...