objective: contradictory results have been reported over microleakage studies of restorative materials and methods. despite the number of publications on leakage there are few evidences comparing the different microleakage evaluation methods. the purpose of the present study was to compare the clearing technique and longitudinal sectioning in the evaluation of dye penetration along a glass fibe...

Three dimensional images were obtained using a single high numerical aperture hologram recorded in a high resolution photoresist with a table top lambda = 46.9 nm laser. Gabor holograms were numerically reconstructed over a range of image planes by sweeping the propagation distance in the numerical reconstruction algorithm, allowing numerical optical sectioning. A robust three dimension image o...

introduction: this study compared the apical sealing ability of a bioceramic based root canal repair filling material (iroot fs) with mineral trioxide aggregate (mta). methods and materials: forty single-canal teeth were prepared and randomly divided into two experimental groups ( n =18) and two control groups ( n =2). teeth in experimental groups were retrofilled with either mta or iroot fs. a...

Quantum optical coherence tomography (QOCT) makes use of an entangled twin-photon light source to carry out axial optical sectioning. We have probed the longitudinal structure of a sample comprising multiple surfaces in a dispersion-cancelled manner while simultaneously measuring the group-velocity dispersion of the interstitial media between the reflecting surfaces. The results of the QOCT exp...

The distribution of viruses and gene therapy vectors is difficult to assess in a living organism. For instance, trafficking in murine models can usually only be assessed after sacrificing the animal for tissue sectioning or extraction. These assays are laborious requiring whole animal sectioning to ascertain tissue localization. They also obviate the ability to perform longitudinal or kinetic s...

Microdamage in bone contributes to the loss of bone quality in osteoporosis and is thought to play a major role in both fragility and stress fractures (Schaffler et al. 1995). In this study, in vivo microcracks in human ribs were bulk-stained in basic fuchsin and viewed in longitudinal section and in 3 dimensions using 2 different computer-based methods of reconstruction: (1) serial sectioning ...

Visualization of individual cutaneous nerve endings has previously relied on laborious procedures of tissue excision, fixation, sectioning and staining for light or electron microscopy. We present a method for non-invasive, longitudinal two-photon microscopy of single nerve endings within the skin of anesthetized transgenic mice. Besides excellent signal-to-background ratio and nanometer-scale ...

OBJECTIVE Contradictory results have been reported over microleakage studies of restorative materials and methods. Despite the number of publications on leakage there are few evidences comparing the different microleakage evaluation methods. The purpose of the present study was to compare the clearing technique and longitudinal sectioning in the evaluation of dye penetration along a glass fiber...