Background: Assisted fertilization procedures are a currently widespread practice to regulate reproduction in humans and animals. The arising question is why the human being manipulating gametes to generate new individuals, if we do not understand yet the molecular mechanism of fertilization?. Successful completion of fertilization in mammals is dependent on three membrane fusion events: 1. the...

introduction: intracellular ph (phi) regulates essentially all aspects of cellular activities. however, it is unknown how endoplasmic reticulum (er) potassium channels sense phi. in this study, we investigate the direct effects of phi on er potassium channels. methods: we used channel incorporation into the bilayer lipid membrane method. l-α-phosphatidylcholine, a membrane lipid, was extracted ...

Phycobiliproteins have been used as a natural dye, an analytical probe for fluorescence studies, and chemical medicinal use. An ultrafiltration membrane is useful the purification of phycobiliproteins. This study addressed phycobiliprotein-containing solution, extracted from Nostoc commune , which was individually performed by two kinds (MWCO 150 kDa 10 kDa) in cross-flow. Three proteins were m...

The mechanism that mediates the interaction between contractile ring and plasma membrane during cytokinesis remains elusive. We previously found ERM (Ezrin/Radixin/Moesin) proteins, which usually mediate cellular pole contraction, become over-accumulated at cell equator support furrow ingression upon loss of other actin-membrane associated anillin supervillin. In this study, we addressed molecu...

Introduction: Intracellular pH (pHi) regulates essentially all aspects of cellular activities. However, it is unknown how endoplasmic reticulum (ER) potassium channels sense pHi. In this study, we investigate the direct effects of pHi on ER potassium channels. Methods: We used channel incorporation into the bilayer lipid membrane method. L-α-phosphatidylcholine, a membrane lipid, was extrac...

seeds deterioration is a natural phenomenon that occurs in all seeds and leading to gradual decline of seed viability during storage. however, the rate at which seeds age depends upon their physiological status, genetic constitution and storage conditions. in the present study we the examined relationship between accumulation of reducing and non-reducing of sugars, soluble protein, the activity...

protein memories are novel technologies which use the hidden abilities of bacteria proteins and biological molecules in order to the electronic processes. four decades before baceriorhdopsin ( br) protein was extracted from the membrane of halobacerinum salina rum found in high salt water depositions after its original discovery in 1967. in this study, for immobilizing the protein a film has be...

One of the barriers during whey filtration using UF membrane is the fouling phenomenon of the membrane, which is caused by whey proteins. In this work, the UF membranes were prepared using polysufone (PSf), dimethyl formamide (DMF), 1 wt.% poly vinyl pyrrolidone (PVP) and different concentrations of LiCl via phase inversion induced by immersion precipitation. The prepared membranes were charact...

Background: Tissue engineering has been investigated as a potential method for healing traumatized tissues. Biomaterials are material devices or implants used to repair or replace native body tissues and organs. The present study was conducted to evaluate the effects of decontamination methods on biological/mechanical properties and degradation/adhesion test of the platelet‑-rich fibrin (PRF) m...