نتایج جستجو برای: multiplex reverse transcriptase pcr rt

تعداد نتایج: 295068  

A. Akhondzadeh Basti, A. Misaghi, D. Al-taghlubee, D. Shayan, H. Gandomi, P. Shayan,

Background: Meat species adulteration has become a problem of concern. This study aimed to compare two previously published multiplex Polymerase Chain Reaction (PCR) methods for meat species authentication.  Methods: The primers used in the first multiplex PCR involved species-specific reverse primer for sheep, goat, cattle, pig, and donkey with universal forward primer. In the second multiple...

پایان نامه :وزارت علوم، تحقیقات و فناوری - دانشگاه الزهراء - دانشکده علوم پایه 1390

باکتری ها کارخانه های بی همتای بیولوژیک در تولید محصولات زیست فناوری هستند ویژگی-هایی نظیر تحمل شرایط خاص رشد و تولید آنزیم های مختلف، باکتریها را برای بهره برداری مورد توجه قرار داده است. عده ای از باکتری ها واجد عوامل ژنتیکی با قابلیت جابه جایی هستند. نظیر اینترون های گروه ii که سه آنزیم با ارزش وارونوشتاز , ( reverse transcriptase) اندونوکلئاز ,(endonuclease) مچوراز (maturase) رارمز می کنن...

Journal: :Facets 2021

Wastewater-based surveillance methods have been implemented in several countries as a tool for monitoring SARS-CoV-2 at community scale. A variety of used concentrating, extracting, and detecting the virus, with no clear consensus on most effective approach. In this note, we report preliminary findings from study that is tracking wastewater Halifax, Nova Scotia, specific focus use four reverse ...

Journal: :Archivum immunologiae et therapiae experimentalis 2003
Simone Mocellin Carlo R Rossi Francesco M Marincola

In the era of the Human Genome Project, quantitation of gene expression by tumor/host cells is of paramount importance to investigate gene patterns responsible for cancer development, progression and response/resistance to treatment. Quantitative real-time PCR (qrt-PCR) technology has recently reached a level of sensitivity, accuracy and practical ease that support its use as a routine bioinstr...

Journal: : 2021

Aim: The aim of the study was to evaluate incidence asymptomatic COVID-19 disease in pregnant women withrectal and oropharyngeal/nasopharyngeal swab compare efficacy two samples.Material Method: This prospective cohort included 234 who had undergoneplanned cesarean section between May 7 September 24, 2020 obstetrics unit a tertiary care center, Ankara,Turkey. swab, rectal placental, amniotic fl...

Journal: :iranian journal of virology 0
e fakhari azad university of zanjan, zanjan, iran m norouzi department of virology, tehran university of medical science, tehran, iran sm jazayeri department of virology, tehran university of medical science, tehran, iran

background and aims: lamivudine is amongst the antiviral for drug chronic hepatitis b treatment. during therapy with lamivudine, variants may emerge with ymdd mutation in the reverse transcriptase (rt) region of polymerase gene. this mutation might have a role in drug resistant for hbv. materials and methods: hbv dna extraction from serum sample of 88 patients, were subjected to nested pcr for ...

Journal: :The American journal of tropical medicine and hygiene 2012
Banugopan Kesavaraju Ary Farajollahi Richard L Lampman Michael Hutchinson Nina M Krasavin Sonya E Graves Sammie L Dickson

The rapid analyte measurement platform (RAMP) system is an immunoassay test for West Nile virus (WNV) detection. Although reverse transcriptase-polymerase chain reaction (RT-PCR) methodology has been regarded as the gold standard for confirming WNV presence, usage of RAMP testing kits has increased in the past years. We collected RAMP test result data that were subsequently confirmed with RT-PC...

Journal: :The Journal of neuroscience : the official journal of the Society for Neuroscience 2004
Bruno Cauli Xin-Kang Tong Armelle Rancillac Nella Serluca Bertrand Lambolez Jean Rossier Edith Hamel

The role of interneurons in neurovascular coupling was investigated by patch-clamp recordings in acute rat cortical slices, followed by single-cell reverse transcriptase-multiplex PCR (RT-mPCR) and confocal observation of biocytin-filled neurons, laminin-stained microvessels, and immunodetection of their afferents by vasoactive subcortical cholinergic (ACh) and serotonergic (5-HT) pathways. The...

2012
Manabu Soda Kazutoshi Isobe Akira Inoue Makoto Maemondo Satoshi Oizumi Yuka Fujita Akihiko Gemma Yoshihiro Yamashita Toshihide Ueno Kengo Takeuchi Young Lim Choi Hitoshi Miyazawa Tomoaki Tanaka Koichi Hagiwara Hiroyuki Mano

Purpose: EML4-ALK is a lung cancer oncogene, and ALK inhibitors showmarked therapeutic efficacy for tumors harboring this fusion gene. It remains unsettled, however, how the fusion gene should be detected in specimens other than formalin-fixed, paraffin-embedded tissue. We here tested whether reverse transcription PCR (RT-PCR)-based detection of EML4-ALK is a sensitive and reliable approach. Ex...

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