Although DNA analysis based on the polymerase chain reaction (PCR) offers potential advantages for screening newborns for sickle cell disease, few data are available concerning the reliability of PCR-based tests for such screening. We describe a protocol for detecting the A, S, and C alleles of the beta-globin gene in dried blood from phenylketonuria screening cards. This method is based on PCR...

In the recent years, there is evidence of training a red type of zebrafish which differs from wild-type in body color. There is not any document how it reaches to the ornamental fish farms of Iran but at first, it was a doubt it belongs to a morphotype or genetic modification (GM). First of all, a set primer was designed to validate zebrafish species. Mitochondrial 16srDNA was selected and ampl...

BACKGROUND Real-time polymerase chain reaction (PCR) is generally regarded as a very accurate and time-saving method, but it is expensive to run. We evaluated the reliability of an inexpensive and a researcher-friendly gel electrophoresis-based PCR method for the quantification of mRNA, and the results were compared with those obtained by real-time PCR. METHODS We compared the results of rela...

BACKGROUND Few studies have addressed the timing of cervical cytologic abnormalities and human papillomavirus (HPV) positivity during the course of an infection. It remains largely unknown how infections detected by HPV and cytology wax and wane relative to each other. The aim of this analysis was to assess the longitudinal relationship of abnormal cytology and HPV positivity in a 7-year prospe...

background: brucellosis is a zoonotic disease and its symptoms and appearances are not exclusive in human and its tradi­tional diagnosis is based on blood culture and serological methods. for more sensitive and specific detection, the pcr method is recommended. methods: one hundred four blood samples were gathered from suspicious patients with brucellosis different ages form ker­manshah, mazand...

We present a protocol for effi cient amplifi cation of a large number of DNA targets using a single-tube polymerase chain reaction (PCR) based on PCR suppression. This method allows amplifi cation of each DNA target with only one target-specifi c primer whereas the other primer is common for all targets. Thus, this approach requires n+1 primers for n targets instead of 2n in conventional PCR an...

background: the aim was to compare three different methods (direct examination, culture and pcr meth­ods) for the diagnosis of acanthamoeba keratitis (ak) in corneal scrapes. methods: twenty eight corneal scrapes and contact lenses were collected from keratitis patients and re­ferred to the de­partment of medical parasitology and mycology, school of public health, tehran univer­sity of medical ...

PURPOSE To develop a new detection and typing method of oculopathogenic strains of subgenus D adenoviruses directly from conjunctival scrapings by a combination of polymerase chain reaction (PCR) and restriction enzyme analysis (REA). METHODS A new PCR method using primer pairs of AF2/AR2, which are specific for the fiber genes, were developed to amplify 1150-bp products from nine oculopathog...