Passenger leukocytes have been demonstrated to play significant roles in initiating and also regulating immune reactions after organ transplantation. Reliable techniques to detect donor leukocytes in recipients after organ transplantation are essential to analyze the role, function, and behavior of these leukocytes. In this report we describe a simple, reliable method to detect donor cells with...

THE finding of megaloblasts in the peripheral blood is often regarded as an uncommon event, confined to patients with severe anaemia. Methods of concenitrating the megaloblasts by making preparations from the "buffy coat'i of the haematocrit have been described (Goodall, 1957, Whitby and Britton, 1957). This paper records our experience of examining the peripheral blood for megaloblasts. METHOD...

Peripheral blood is the chief source of mononuclear cells (lymphocytes and monocytes) for immunologic studies of humans. This appendix presents protocols for obtaining blood by simple venipuncture when relatively small amounts of blood (10 to 100 ml) are necessary or by lymphapheresis when large amounts (300 to 5000 ml) are necessary. Cells collected by these procedures can be further separated...

A B S T R A C T By using a methylcellulose clonal assay, we cultured peripheral blood erythropoietic precursors from a patient with sickle cell anemia, a patient with sickle cell hemoglobin C disease, and a normal volunteer. We then analyzed the synthetic rates ofadult and fetal hemoglobins (Hb) in individual erythropoietic bursts. Hb were labeled with '4C-amino acids in culture, separated by s...

introduction: although extensive research has been conducted on lung cancer markers, a singular clinically applicable marker has not been found yet. the objective of this study was to evaluate the sensitivity and the specificity of carcinoembryonic antigen (cea) mrna and lung-specific x protein (lunx) mrna biomarkers in peripheral blood to detect lung cancer individually and simultaneously. met...

background : mdm2, e-cadherin, survivin and her2 genes are involved in the regulation of normal cell growth. however, any crucial change in their expression levels can convert a normal cell into a cancer cell. numerous studies have identified alterations of these gene expression levels in various cancers, particularly in breast cancer. thus, they may be used as diagnostic biomarkers. in this ex...

Background: Human peripheral blood NK cells constitutively express CD56 and CD16 antigens. Peripheral blood NK cells seem to be strongly related with decidual NK cells, and may reflect the decidual NK cell functional status. There are varied reports concerning the relationship between NK cell cytotoxicity in women with recurrent spontaneous abortion. Objective: To study NK activity in women wit...