in order to study the periplasmic expression of human growth hormone (hgh) in escherichia coli, the related cdna was inserted in two expression plasmids carrying pelb signal peptide, one with lac bacterial promoter and the other with a bacteriophage t7-based promoter. the recombinant plasmids were moved to tg1 and bl21 strains of e. coli, respectively. to induce the expression systems, iptg and...

The influence of moisture sorption on spin–spin (T2) relaxation times for skim milk powder (SMP), protein concentrates (MPC50 and MPC80) amorphous lactose was studied by low-field nuclear magnetic resonance (NMR). For MPC80, only one main peak observed in the T2 distribution, whereas SMP MCP50, two peaks, relating to water associated with protein, were at aw <0.4; >0.4 crystallised no visible s...

Milk and milk derivatives are widely consumed because of their high nutritional density. Donkey products have been since ancient times. The use donkey in the human diet is gaining popularity. abundance antibacterial components protective elements sets it apart from other animals. Like milk, has low fat, lactose, casein/whey protein ratio. whey protein's anti-proliferative properties imply lung ...

The genes coding for the lactose permease and beta-galactosidase, two proteins involved in the metabolism of lactose by Lactobacillus bulgaricus, have been cloned, expressed, and found functional in Escherichia coli. The nucleotide sequences of these genes and their flanking regions have been determined, showing the presence of two contiguous open reading frames (ORFs). One of these ORFs codes ...

selenium (se) is considered as an essential element for human body. selenized probiotic fermented milk drink (doogh) as a functional beverage to increase se available in body. the predominate sugar, protein and mineral ion of iranian yoghurt drink (doogh) are lactose, casein and calcium, respectively. different levels of  selenium (2.5 – 3- 3,5-  4 and 4.5) is prepared and its suitability based...

The apparent instability of beta-galactosidase in toluene-treated cells or cell-free extracts of lactic streptococci is explained by the fact that these organisms do not contain the expected enzyme. Instead, various strains of Streptococcus lactis, S. cremoris, and S. diacetilactis were shown to hydrolyze o-nitrophenyl-beta-d-galactoside-6-phosphate (ONPG-6-P), indicating the presence of a diff...

Chicory plant (Dorema aucheri) belongs to Asteraceae family and contains flavonoids. Milk is a mixture of protein, fat, lactose, minerals. This research carried out in order to evaluate the effect of chicory plant on the activities of bifidobacterium bifidum and lactobacillus acidophilus. Four containers containing a liter of 1.5 % sterilized skim milk were s...

In order to study the periplasmic expression of human growth hormone (hGH) in Escherichia coli, the related cDNA was inserted in two expression plasmids carrying pelB signal peptide, one with lac bacterial promoter and the other with a bacteriophage T7-based promoter. The recombinant plasmids were moved to TG1 and BL21 strains of E. coli, respectively. To induce the expression systems, IPTG and...

Lactose and neuramin lactose were produced by incubating subcellular particles from rat mammary gland in a system containing glucose, uridine diphosphate galactose, and cytidine monophosphate N-acetylneuraminic acid. The K, values for glucose in the synthesis of neuramin lactose were fairly constant for three similarly prepared enzyme preparations (0.25, 0.17, and 0.22 m&r), but were widely dis...