Protein kinase C (PKC), a multi-gene family of enzymes, plays key roles in the pathways of signal transduction, growth control and tumorigenesis. Variations in the intracellular localization of the individual isoforms are thought to be an important mechanism for the isoform-specific regulation of enzyme activity and substrate specificity. To provide a dynamic method of analyzing the localizatio...

OBJECTIVES The aim of this study was to analyze modifications in the plasma protein map during an acute coronary syndrome (ACS) using proteomics. BACKGROUND Proteomics is a new technology that allows the detection and identification of several proteins at a given time in a sample. METHODS Plasma from 19 patients, 11 with acute myocardial infarction (AMI) and 8 with unstable angina (UA), was...

The response to glucocorticoids (GCs) therapy classifies severe refractory asthma (SRA) and mild asthma, so the glucocorticoid receptors (GCRs) gene expression may be involved in SRA pathogenesis. Thus, it is aimed to compare the expression levels of two GCR isoforms (GCRα and GCRβ) in SRA, mild asthmatics, and healthy controls. Total RNA was isolated from the peripheral blood mononuclear lymph...

objective(s):psoriasis is an autoimmune disease that appears on the skin. although psoriasis is clinically and histologically well characterized, its pathogenesis is unknown in detail. the aims of this study were to evaluate the proteome of psoriatic patients' sera and to compare them with those of normal healthy human to find valuable biomarkers. materials and methods: in a case-control s...

In higher eukaryotes many genes encode protein isoforms whose properties and biological roles are often poorly characterized. Here we describe systematic approaches for detection of either distinct isoforms, or separate pools of the same isoform, with differential biological properties. Using information from ion intensities we have estimated protein abundance levels and using rates of change i...

Progesterone and its interaction with nuclear progesterone receptors (PR), PR-A and PR-B, play a critical role in the regulation of female reproductive function in all mammals. However, our knowledge of the regulation and possible cellular function of PR protein isoforms in the Fallopian tube and uterus in vivo is still very limited. In the present study, we revealed that equine gonadotropin (e...

Nitric oxide (NO) is an essential regulator of Drosophila development and physiology. We describe a novel mode of regulation of NO synthase (NOS) function that uses endogenously produced truncated protein isoforms of Drosophila NOS (DNOS). These isoforms inhibit NOS enzymatic activity in vitro and in vivo, reflecting their ability to form complexes with the full-length DNOS protein (DNOS1). Tru...

Progesterone and its interaction with nuclear progesterone receptors (PR) PR-A and PR-B play a critical role in the regulation of female reproductive function in all mammals. However, our knowledge of the regulation and possible cellular function of PR protein isoforms in the fallopian tube and uterus in vivo is still very limited. In the present study, we revealed that equine chorionic gonadot...

apoptosis, a form of programmed cell death that occurs under physiological as well as pathological conditions, is characterized by morphological and biochemical features. while the importance of caspases in apoptosis is established, several noncaspase proteases (ca2+-dependent proteases) such as calpain may play a role in the execution of apoptosis. the calpain family consists of two major isof...

The understanding of intracellular signaling pathways and their physiological effects has been confounded by the existence of numerous isoforms of the various signaling components. Thus, many families of protein kinases comprise several subfamilies, each of which may contain multiple isoforms deriving from distinct genetic loci. Furthermore, each locus may produce multiple products through alte...