previous studies have indicated that fish oil intake wi1l improve semen quality in some mammals and birds. the aim of this study was to investigate the effect of dietary fish oil on reproductive performance in ram. eight zandi rams were divided into two groups and fed either a control diet or a supplemented diet with fish oil. both of the diets were isocaloric and isonitrogenous and formulated ...

Background: The success of fertilization is directly associated with semen quality and the sperm preparation. Considering the common use of cryopreserved spermatozoa, there is a need to develop strategies for sperm preparation in order to achieve a sperm sample of high quality through a rigorous selection of sperm. Thus, sperm cells are being more extensively investigate. This study aimed evalu...

experiment was conducted to study the effect of different antibiotics on sperm quality of ram by a completely randomized block design using 23 factorial arrangement with 2 block and 4 observations in each experimental units. factors include three antibiotics named gentamycin (g), lincomycin (l) and ampicillin (a) that were used at two different levels including zero and, respectively 5, 10 and ...

The present study was conducted to detect sperm apoptosis in fresh, capacitated and frozen ram semen and to determine its relationship with other seminal parameters as motility and viability. The cryopreservation process induces the plasma membrane disruptions, attended with increasing of phoshpatidylserine molecules expression on the extracellular surface, wherefore some of the live spermatozo...

Sperm mediated gene transfer can be an inexpensive and simple method in animal transgenesis; however its efficiency is poor, mainly due to the spermatozoa’s lesser uptake of exogenous DNA. In the present study, the effects of lipofection and other augmentation techniques, such as sperm freezing and spermatozoa treatment with triton X100 and DMSO, on exogenous DNA uptake by sheep spermatozoa and...

OBJECTIVE The effects of dietary fish oil on semen quality and sperm fatty acid profiles during consumption of n-3 fatty acids as well as the persistency of fatty acids in ram's sperm after removing dietary oil from the diet were investigated. MATERIALS AND METHODS In this experimental study, we randomly assigned 9 Zandi rams to two groups (isoenergetic and isonitrogenous diets): control (CTR...

Cellular prion protein (Prp(C)) is a glycoprotein usually associated with membranes via its glycosylphosphatidylinositol (GPI) anchor. The trans-conformational form of this protein (Prp(SC)) is the suggested agent responsible for transmissible neurodegenerative spongiform encephalopathies. This protein has been shown on sperm and in the reproductive fluids of males. Antibodies directed against ...

Background: Spermatozoa preservation is an approach to improve the fertility rate to pass on the valuable genetic material from sire to their offspring. During the last 20 years, reproductive biotechnologists have focused on the approaches that improve spermatozoa cryosurvival. One of the possible mechanisms is supplementation in semen extender. Objectives: The aim of this study was to evaluat...